Live Cell Imaging

  • High speed multi-modal imaging with optical sectioning with 2 pinhole sizes
  • Four laser lines: 405nm, 488nm, 561nm, 637nm
  • Environmental control for samples which need heating and CO2
  • Microscope autofocus control for time-lapse imaging
  • Additional technologies: TIRF, SRRF, point localization super-resolution, MicroPoint laser ablation, and Mosaic3 photostimulation
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Andor XD Revolution Spinning Disk Confocal
  • High-speed multidimensional imaging with optical sectioning
  • Spinning disk modality produces high SNR images with minimal photobleaching
  • High sensitivity EMCCD camera
  • Laser lines for all classes of fluorscent proteins
  • Incubation for mammalian cells
  • IR reflection based autofocus
  • Silicon oil 60x objective for RI match
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DeltaVision Elite Deconvolution Microscope
  • Widefield imaging of living and fixed samples
  • Optimized deconvolution
  • Solid-state illumination, fast excitation switching
  • Temperature, humidity, CO2 control
  • Hardware-based autofocus
  • High-precision XYZ stage
  • Dual cameras - high-resolution and high-sensitivity
  • 405 nm and 488 nm lasers for photoactivation and photo-bleaching experiments
  • Optical axis integration/z-sweep acquisition
  • Kohler and critical illumination
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Live Cell Station 1: Zeiss Axio Observer
  • Imaging living samples and fixed samples - any fluorescent protein
  • Temperature, humidity, CO2 control
  • High sensitivity - EMCCD camera
  • Multiposition timelapse
  • Tiled images
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Olympus VivaView FL Incubator Microscope-2019
  • Incubation system allowing multi-day timelapse
  • Fluorescence and transmitted light DIC imaging
  • Up to 8 samples can be imaged per timelapse
  • Multiposition timelapse
  • Z-stacks
  • Liquid handling - change media or add drugs
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