Andor Dragonfly Spinning Disk Confocal plus

Location
LSRC C367Capabilities
- High speed multi-modal imaging with optical sectioning with 2 pinhole sizes
- Four laser lines: 405nm, 488nm, 561nm, 637nm
- Environmental control for samples which need heating and CO2
- Microscope autofocus control for time-lapse imaging
- Additional technologies: TIRF, SRRF, point localization super-resolution, MicroPoint laser ablation, and Mosaic3 photostimulation
Cost
$17.00 per hourDetailed Specifications
Scanhead
- Andor Dragonfly 505 unit with Borealis illumination spinning disk confocal - 2 pinhole sizes (25 um and 40 um)
- TIRF microscopy via a separate lightpath
- Motorized dichroics - BGR (405/488/561/640) and CY (445/515)
Additional technology on system:
- TIRF microscopy via a separate lightpath
- iXon Life 888 EMCCD camera-based super-resolution radial fluctuation (SRRF)
- deconvolution - post imaging or during acquisition
- point localization dSTORM super-resolution image acquisition
Lasers:
- Andor AOTF control
- 405 nm (100 mw) diode
- 488 nm (150 mW) diode
- 561 nm (100 mW) diode
- 637 nm (140 mW) diode
Filters (both camera light paths):
- Blue: 450/50
- Cyan: 478/37
- Green: 525/50
- Yellow:540/30
- Red: 600/50
- Far Red: 700/75
- Beam Splitter: 570LP (dual-camera mode)
Microscope:
- Leica DMi8
- ASI Motorized stage with linear encoding
- 300 micron piezo for rapid z-control
- Autofocus - touchpad or software controlled
- LED transmitted light - CoolLED
- CoolLED - 3 color fluorescence illumination for blue, green, and red channels
- OKO Labs enclosure chamber with heat and CO2 control
- OKO Labs sample adapters for slides, 35mm dishes, and multiwell plates
Objectives:
- 10x/0.45 HC PL APO PH1 (Leica 11506411), Dry, WD: 1.80 mm
- 20x/0.75 HC PL APO CS2 (Leica 11506517), Dry, WD: 0.62 mm
- 40x/1.3 HC PL APO CS2 (Leica 11506358), Oil, WD: 0.24 mm
- 63x/1.47 TIRF HC PL APO CORR (Leica 11506319), Oil, WD: 0.10 mm
- 63x/1.20 W HC PL APO CORR CS2 (Leica11506346), Water, WD: 0.30 mm
- 100x/1.40-0.70 HCX PL APO (Leica 11506210), Oil, WD: 0.09mm
Zoom Optics (for both cameras):
- 1X
- 1.5X
- 2X
Calibrations:
- 10x = 1.21um/pixel (EMCCD); 0.61um/pixel (Zyla)
- 20x = 0.61um/pixel (EMCCD); 0.31um/pixel (Zyla)
- 40x oil = 0.30um/pixel (EMCCD); 0.15um/pixel (Zyla)
- 63x oil (TIRF) = 0.19um/pixel (EMCCD); 0.10um/pixel (Zyla)
- 63x water = 0.19um/pixel (EMCCD); 0.10um/pixel (Zyla)
- 100x oil = 0.12um/pixel (EMCCD); 0.06um/pixel (Zyla)
Cameras and control:
- Andor iXon Life 888 1024x1024 EMCCD, pixel size: 13um
- Andor Zyla PLUS 4.2 Megapixel sCMOS 2048x2043, pixel size: 6.5um
Computer:
Dell Precision T7910 with Intel Xeon CPU ES-2637 v3 @ 3.50 GHz, 96.0 GB memory, and 64-bit Windows 7 Professional. Users' data can be temporarily stored on 4 x 1TB SSDs (in RAID 0) or on a separate 2TB SSD drive.