Andor Dragonfly Spinning Disk Confocal plus

Andor Dragonfly Spinning Disk Confocal plus

User Manual(s)

Location

LSRC C367

Capabilities

  • High speed multi-modal imaging with optical sectioning with 2 pinhole sizes
  • Four laser lines: 405nm, 488nm, 561nm, 637nm
  • Environmental control for samples which need heating and CO2
  • Microscope autofocus control for time-lapse imaging
  • Additional technologies: TIRF, SRRF, point localization super-resolution, MicroPoint laser ablation, and Mosaic3 photostimulation

Cost

$18.00 per hour

Detailed Specifications

Scanhead

  • Andor Dragonfly 505 unit with Borealis illumination spinning disk confocal - 2 pinhole sizes (25 um and 40 um)
  • TIRF microscopy via a separate lightpath
  • Motorized dichroics - BGR (405/488/561/640) and CY (445/515)

Additional technology on system:

  • TIRF microscopy via a separate lightpath
  • iXon Life 888 EMCCD camera-based super-resolution radial fluctuation (SRRF)
  • deconvolution - post imaging or during acquisition
  • tile scanning and stitching
  • point localization dSTORM super-resolution image acquisition

Lasers:

  • Andor AOTF control
  • 405 nm (100 mw) diode
  • 488 nm (150 mW) diode
  • 561 nm (100 mW) diode
  • 637 nm (140 mW) diode

Filters (both camera light paths):

  • Blue: 450/50
  • Cyan: 478/37
  • Green: 525/50
  • Yellow:540/30
  • Red: 600/50
  • Far Red: 700/75
  • NIR/IR: 736nm/LP (only available for the iXon Life 888 EMCCD camera)
  • Beam Splitter: 570LP (dual-camera mode)

Microscope:

  • Leica DMi8
  • ASI Motorized stage with linear encoding
  • 300 micron piezo for rapid z-control
  • Autofocus - touchpad or software controlled
  • LED transmitted light - CoolLED
  • CoolLED - 3 color fluorescence illumination for blue, green, and red channels 
  • OKO Labs enclosure chamber with heat and CO2 control
  • OKO Labs sample adapters for slides, 35mm dishes, and multiwell plates

Objectives:

  • 10x/0.45 HC PL APO PH1 (Leica 11506411), Dry, WD: 1.80 mm
  • 20x/0.75 HC PL APO CS2 (Leica 11506517), Dry, WD: 0.62 mm
  • 40x/1.3 HC PL APO CS2 (Leica 11506358), Oil, WD: 0.24 mm
  • 63x/1.47 TIRF HC PL APO CORR (Leica 11506319), Oil, WD: 0.10 mm
  • 63x/1.20 W HC PL APO CORR CS2 (Leica11506346), Water, WD: 0.30 mm
  • 100x/1.40-0.70 HCX PL APO (Leica 11506210), Oil, WD: 0.09mm

Zoom Optics (for both cameras):

  • 1X
  • 1.5X
  • 2X

Calibrations:

  • 10x = 1.21um/pixel (EMCCD); 0.61um/pixel (Zyla)
  • 20x = 0.61um/pixel (EMCCD); 0.31um/pixel (Zyla)
  • 40x oil = 0.30um/pixel (EMCCD); 0.15um/pixel (Zyla)
  • 63x oil (TIRF) = 0.19um/pixel (EMCCD); 0.10um/pixel (Zyla)
  • 63x water = 0.19um/pixel (EMCCD); 0.10um/pixel (Zyla)
  • 100x oil = 0.12um/pixel (EMCCD); 0.06um/pixel (Zyla)

Cameras and control:

  • Andor iXon Life 888 1024x1024 EMCCD, pixel size: 13um
  • Andor Zyla PLUS 4.2 Megapixel sCMOS 2048x2043, pixel size: 6.5um

Computer: 

Dell Precision T7910 with Intel Xeon CPU ES-2637 v3 @ 3.50 GHz, 96.0 GB memory, and 64-bit Windows 7 Professional. Users' data can be temporarily stored on 4 x 1TB SSDs (in RAID 0) or on a separate 2TB SSD drive.