Andor Dragonfly (505) Spinning Disk Confocal Plus

Andor Dragonfly Spinning Disk Confocal plus

User Manual(s)

Location

LSRC C136-B

Capabilities

  • High speed multi-modal imaging with optical sectioning with 25um and 40um pinhole sizes
  • Four laser lines: 405nm, 488nm, 561nm, 637nm
  • Environmental control for samples which need heating and CO2
  • Hardware autofocus control for time-lapse imaging
  • Additional technologies: TIRF, SRRF, point localization super-resolution, MicroPoint laser ablation, and Mosaic 3 photostimulation

Cost

$32.00 per hour

Detailed Specifications

Scanhead

  • Andor Dragonfly 505 unit with Borealis illumination spinning disk confocal - 2 pinhole sizes (25 um and 40 um)
  • TIRF microscopy via a separate lightpath
  • Motorized dichroics - BGR (405_13nm/488_13nm/561_6nm/640_16nm) and CY (405_13nm/445_13nm/514_14nm/640_16nm)

Additional technology on system:

  • TIRF microscopy via a separate lightpath
  • iXon Life 888 EMCCD camera-based super-resolution radial fluctuation (SRRF)
  • deconvolution - post imaging or during acquisition
  • tile scanning and stitching
  • point localization dSTORM super-resolution image acquisition

Lasers:

  • Andor AOTF control
  • 405 nm (100 mw) diode
  • 488 nm (150 mW) diode
  • 561 nm (100 mW) diode
  • 637 nm (140 mW) diode

Filters (both camera light paths):

  • Blue: 450/50
  • Cyan: 478/37
  • Green: 525/50
  • Yellow:540/30
  • Red: 600/50
  • Far Red: 700/75
  • NIR/IR: 736nm/LP (only available on the iXon Life 888 EMCCD camera)
  • Beam Splitter: 570LP (dual-camera mode)

Microscope:

  • Leica DMi8
  • ASI Motorized stage with linear encoding
  • 300 micron piezo for rapid z-control
  • Autofocus - touchpad or software controlled
  • LED transmitted light - CoolLED
  • CoolLED - 3 color fluorescence illumination for blue, green, and red channels
  • OKO Labs enclosure chamber with heat and CO2 control
  • OKO Labs sample adapters for slides, 35mm dishes, and multiwell plates

Objectives:

  • 10x/0.45 HC PL APO PH1 (Leica 11506411), Dry, WD: 2.80 mm
  • 20x/0.75 HC PL APO CS2 (Leica 11506517), Dry, WD: 0.62 mm
  • 40x/1.3 HC PL APO CS2 (Leica 11506358), Oil, WD: 0.24 mm
  • 63x/1.47 TIRF HC PL APO CORR (Leica 11506319), Oil, WD: 0.10 mm
  • 100x/1.40-0.70 HCX PL APO (Leica 11506210), Oil, WD: 0.09mm
  • 63x/1.30, HCX PL APO (Leica 11506193), Glycerol, WD: 0.28mm

          (Available: 63x/1.20 W HC PL APO CORR CS2 (Leica11506346), Water, WD: 0.30 mm)

Zoom Optics (for both cameras):

  • 1X
  • 1.5X
  • 2X

Calibrations:

  • 10x = 1.21um/pixel (EMCCD); 0.61um/pixel (Zyla)
  • 20x = 0.61um/pixel (EMCCD); 0.31um/pixel (Zyla)
  • 40x oil = 0.30um/pixel (EMCCD); 0.15um/pixel (Zyla)
  • 63x oil (TIRF), water, glycerol = 0.19um/pixel (EMCCD); 0.10um/pixel (Zyla)
  • 100x oil = 0.12um/pixel (EMCCD); 0.06um/pixel (Zyla)

Cameras and control:

  • Andor iXon Life 888 1024x1024 EMCCD, pixel size: 13um
  • Andor Zyla PLUS 4.2 Megapixel sCMOS 2048x2043, pixel size: 6.5um

Computer: 

HP Z8 Fury G5 Workstation Desktop PC running Windows 11 Pro (64 bit), Intel Xeon W5-3435X (3.10GHz) Processors, 128GB RAM memory, 16TB NVMe M.2 hard drives (4 drives in RAID 0) for data storage, dual Nvidia RTX Pro 4000 Blackwell 24GB graphics cards

(Retired in 08-2026: Dell Precision T7910 with Intel Xeon CPU ES-2637 v3 @ 3.50 GHz, 96.0 GB memory, and 64-bit Windows 7 Professional. Users' data can be temporarily stored on 4 x 1TB SSDs (in RAID 0) or on a separate 2TB SSD drive.)