The Dragonfly spinnign disk confocal microscope has been upgraded to Fusion 2.7. The new Fusion has greatly improved the workflow for ‘Montage’ (tile-scanning) setup. It now also provides ‘Focus Map’, which allows focus setup along an undulating tissue section and ensures the stitched image to be in good focus all through it. In addition, Fusion 2.7 includes in it a ‘Mosaic’ module for photostimulation, photoactivation, photobleaching, etc.. A 445nm diode laser is used for those purposes.Please note, user's own… read more about Fusion 2.7 is now on the Dragonfly Spinning Disk Confocal »
An Oko-Lab BoldLine 3 incubator has been added to the Leica Stellaris 8 in C142 of LSRC. This incubator features tubeless heating, and direct reading and closed-feedback control of temperature, CO2 and humidity in the sample chamber. Multi-hour live cell imaging on the Stellaris 8 is now possible. Sample adapters are available for single 35mm dish, chambered coverslip, regular slide, and multiwell plate. The enclosure also has a LED light built inside. Using the foot pedal under the air table conveniently switches it… read more about Oko-Lab BoldLine 3 incubator is added to Leica Stellaris 8 »
The Duke LMCF has several different super-resolution techniques available. Here we show our Zeiss ELRYA7 which can perform SIM (structured illumination microscopy) and point localization along with live cell imaging and Apotome. SIM resolution can reach 120nm in x,y. We would be glad to help you learn to utilize this system - just get in touch with the LMCF staff. lmcf@duke.edu In addition, we have other super-resolution techniques including Leica STED (stimulated emission depletion), Airyscan and SRRF (super-… read more about Super-resolution available in the LMCF »