The new Dragonfly 200 Spinning Disk Confocal is installed in MSRB3 and is ready for new users. The system has 405, 488, 561, 637, and 730 nm lasers, coupled with 25 and 40 μm pinhole disks and two camera options.A high-resolution SONA 6 sCMOS camera with 95% quantum efficiency and Super-resolution radial fluctuations (SRRF) technology to resolve details in the 100nm range. A high-sensitivity iXon Life 888 EMCCD camera for single photon sensitivity ideal for low signal samples.The system also… read more about New Andor Dragonfly 200 Spinning Disk Confocal in MSRB3 »
The Leica Stellaris 8 confocal microscope has been updated to LAS X 4.9.2 that comes with 'AI Denoise' function. It can be turned on separately for individual channels to improve spatial and temporal signal and contrast of images. 'AI Denoise' benefits all types of samples, but it is specifically useful for, at least, samples with weak signals, live samples for time-lapse imaging where using high-does lasers hurts the cells, samples that are imaged at high scanning speed (therefore poorer contrast), and samples for 'Montage… read more about 'AI-Denoise' is added to the Leica Stellaris 8 »
The Dragonfly spinning disk confocal microscope in C136B of LSRC now has a new more powerful computer. It is a HP Z8 Fury G5 Workstation Desktop PC that runs Windows 11 Pro (64 bit). Intel Xeon W5-3435X (3.10GHz) is the processor. Total of 128GB RAM memory and 16TB NVMe M.2 hard drives (4 drives in RAID0) for data storage are installed on it. Dual Nvidia RTX Pro 4000 Blackwell 24GB graphics cards are the main powerhouse for image acquisition and procession. All other major microscopy hardware and the program Fusion 2.7… read more about Dragonfly has a new computer »
The Duke LMCF has several different super-resolution techniques available. Here we show our Zeiss ELRYA7 which can perform SIM (structured illumination microscopy) and point localization along with live cell imaging and Apotome. SIM resolution can reach 120nm in x,y. We would be glad to help you learn to utilize this system - just get in touch with the LMCF staff. lmcf@duke.edu In addition, we have other super-resolution techniques including Leica STED (stimulated emission depletion), Airyscan and SRRF (super-… read more about Super-resolution available in the LMCF »