LMCF has relocated the Zeiss PALM MicroBeam laser capture microdissection microscope to MSRB3 4221, next door from its original location. Please contact LMCF@duke.edu if you have any questions about the microscope and the laser capture microdissection technology. read more about Zeiss Laser Capture Microdissection Microscope is now in MSRB3 4221 »
After about 17 years of serivce, the Olympus VivaView incubator fluorescent microscppe started to have issues. Long-time live cell imaging on it deems to be unreliable. LMCF has made a hard decision to retire it. Most of its functionality can be found on the Live Cell Station (a Zeiss Axio Observer Z1) in C142 of LSRC. Actually, the latter has more capabilities than the VivaView in various aspects. For example, the Live Cell Station has more choice of objective lenses and more channels, and it takes live samples in more… read more about Olympus VivaView Incubator Microscope Retired »
The Dragonfly spinnign disk confocal microscope has been upgraded to Fusion 2.7. The new Fusion has greatly improved the workflow for ‘Montage’ (image tile-scanning) setup. It now also provides ‘Focus Map’, which allows focus setup along an undulating tissue section and ensures good focus all through the stitched image. In addition, Fusion 2.7 includes in it a ‘Mosaic’ module for photostimulation, photoactivation, photobleaching, etc.. A 445nm diode laser is used for those purposes.Please note, user's own channel… read more about Dragonfly Spinning Disk Confocal Has Been Updated to Fusion 2.7 »
The Duke LMCF has several different super-resolution techniques available. Here we show our Zeiss ELRYA7 which can perform SIM (structured illumination microscopy) and point localization along with live cell imaging and Apotome. SIM resolution can reach 120nm in x,y. We would be glad to help you learn to utilize this system - just get in touch with the LMCF staff. lmcf@duke.edu In addition, we have other super-resolution techniques including Leica STED (stimulated emission depletion), Airyscan and SRRF (super-… read more about Super-resolution available in the LMCF »