Glass Coverslip Cleaning (Acid Wash)

A clean coverslip will help cell adhesion and enhance polyamino acids coating.

  1. Make 1M HCl in a glass container and put coverslips in it.
  2. Heat the acid to 50-60 °C for 4-16 hours with occasional agitation.
  3. Wash the coverslips extensively in distilled water (two times), then double distilled water (two times). Be sure to wash out the acid between sticked coverslips.
  4. Rinse coverslips in 100% ethanol and dry them between sheets of whatman filter paper.
  5. Keep coverslip in a clean container.