The facility has a large variety of laser lines, excitiation and emission filters available across a wide range of microscopes. This table is to help you quickly find the microscopes able to image a specific fluorophore. For further details see the specification page for each scope (links on the menu on the left).
|Laser line (nm)||Confocal system(s) on which it is available|
|405||510 inverted, SP5s, SP8, 710, 780s and TIRF. Deltavision point activation laser|
|458||510s, SP5s, SP8, 710, 780s|
|477||510s, SP5s, 710, 780s|
|488||All confocals, SD and TIRF. Deltavision point activation/bleaching laser|
|514||510s, SP5s, SP8, 710, 780s, SD|
|561||510 inverted, SP5s, SP8, 710, 780s, SD and TIRF|
|594||510 inverted, 710 and SP5 inv|
|633||All scanning confocals|
Numerous emission filters are available for the confocals and are described on the individual equipment pages. Spectral detection is available on the SP5/SP8 (serial) and 710/780 (parralel).
|DAPI||Live cell, DeltaVision and Axio Imager/Observer|
|CFP||Live cell, DeltaVisio and Axio Imager/Observer|
|GFP||Live cell, DeltaVisio and Axio Imager/Observer|
|YFP||Live cell, DeltaVisio and Axio Imager/Observer|
|Red||Live cell, DeltaVisio and Axio Imager/Observer|
|Far-red||Axio Imager (filterwheel) / Observer (available cube)|
|Cy7||Have cube (generally with Axio Observer)|
Exact wavelengths of the filters are available on the individual equipment pages.