The facility has a large variety of laser lines, excitiation and emission filters available across a wide range of microscopes. This table is to help you quickly find the microscopes able to image a specific fluorophore. For further details see the specification page for each scope (links on the menu on the left).
Laser line (nm) | Confocal system(s) on which it is available |
405 | 510 inverted, SP5s, SP8, 710, 780s and TIRF. Deltavision point activation laser |
445 | SD |
458 | 510s, SP5s, SP8, 710, 780s |
477 | 510s, SP5s, 710, 780s |
488 | All confocals, SD and TIRF. Deltavision point activation/bleaching laser |
496 | Leica SP5s |
514 | 510s, SP5s, SP8, 710, 780s, SD |
543 | 510 upright |
561 | 510 inverted, SP5s, SP8, 710, 780s, SD and TIRF |
594 | 510 inverted, 710 and SP5 inv |
633 | All scanning confocals |
635 | TIRF |
680-1080 | Multiphoton |
Numerous emission filters are available for the confocals and are described on the individual equipment pages. Spectral detection is available on the SP5/SP8 (serial) and 710/780 (parralel).
Widefield system | |
DAPI | Live cell, DeltaVision and Axio Imager/Observer |
CFP | Live cell, DeltaVisio and Axio Imager/Observer |
GFP | Live cell, DeltaVisio and Axio Imager/Observer |
YFP | Live cell, DeltaVisio and Axio Imager/Observer |
Red | Live cell, DeltaVisio and Axio Imager/Observer |
Far-red | Axio Imager (filterwheel) / Observer (available cube) |
Cy7 | Have cube (generally with Axio Observer) |
Exact wavelengths of the filters are available on the individual equipment pages.