Overlaying fluorscence and DIC
- Open the images in ImageJ
- Adjust the contrast if neceesary: Image/Adjust/BrightnessConstrast
- Change or check all Images are in 8-bit format. Image/Type/8-bit
- Image/Color/Merge Channels and the Merge Channels box will appear
- Select the fluorescent images in the appropriate R, G and B channels and the DIC or similar image in gray channel. Select “Create Composite” and click “OK”
- Click on the new ‘composite’ image to activate it
- Image/Type/RGB Color
- Save it as “Tiff” file