Several types of slide based culture systems are available. If you use these you should be aware that they fall into two different groups
- Where you grow the cells on a coverslip thickness piece of glass
- Where you grow the cells on a slide thickness piece of glass and later add a coverslip
These can be convenient for some applications but in general we don't recommend the second group because of the following problem:
The approach may be successful for low magnification/NA objectives with longer working distance