Andor XD Revolution Spinning Disk Confocal

Andor XD Revolution Spinning Disk Confocal

User Manual(s)

Location

LSRC C142

Capabilities

  • High-speed multidimensional imaging with optical sectioning
  • Spinning disk modality produces high SNR images with minimal photobleaching
  • High sensitivity EMCCD camera
  • Laser lines for all classes of fluorscent proteins
  • Incubation for mammalian cells
  • IR reflection based autofocus
  • Silicon oil 60x objective for RI match

Cost

$25 per hour

Detailed Specifications

Scanhead

  • Yokogawa CsuX-1 5000 rpm
  • Andor precision control and laser synchronization
  • Motorized dichroics - BGR (405/488/561) and CYR (445/515/561)

Lasers:

  • Andor AOTF control
  • 445 nm (40 mW) diode
  • 488 nm (50 mW) diode
  • 515 nm (50 mW) diode
  • 561 nm (50 mW) diode

Filters:

  • 440/521/607/700
  • 464/547
  • 447/60
  • 483/32
  • 525/30
  • 542/27
  • 607/36
  • Polarizer
  • LP568

Microscope:

  • IX81 motorized stand
  • ASI Motorized stage with linear encoding
  • 300 micron piezo for rapid z-control
  • Zero-drift compensation reflection based autofocus
  • BGR widefield fluorescence with metal halide source
  • Tokai Hit stage top incubation with CO2
  • Precision Plastics environmental chamber with heating and humidity control

Objectives:

  • 10x/0.3 UPlanFl N dry, WD: 10mm, FN26.5, UIS2
  • 20x/0.5 UplanFl N dry, WD: 2.1mm, ∞/0.17/FN26.5, UIS2
  • 40x/1.3 UPlanFl N Oil, WD: 0.20mm, ∞/0.17/FN26.5, UIS2
  • 60x/1.3 Silicon oil UPlanSApo DIC, WD: 0.30mm, 0.15-0.19/FN26.5, UIS2
  • 60x/1.2 W corr UPlanApo, WD: 0.28mm, 0.13-0.21/FN26.5, UIS2
  • 100x/1.4 oil U PlanSApo DIC, WD: 0.13mm, ∞/0.17/FN26.5, UIS2

Calibrations:

  • 10x = 1.140 µm/pixel
  • 20x = 0.568 µm/pixel
  • 40x oil = 0.283 µm/pixel
  • 60x silicon = 0.189 µm/pixel
  • 60x water = 0.185 µm/pixel
  • 100x oil = 0.112 µm/pixel

(Calibrations with the previous iXon 3 897 EMCCD camera

  • 10x = 1.401 µm/pixel
  • 20x = 0.701 µm/pixel
  • 40x oil = 0.359 µm/pixel
  • 60x silicon = 0.237 µm/pixel
  • 60x water = 0.237 µm/pixel
  • 100x oil = 0.139 µm/pixel)

Cameras and control:

  • Andor iXon Life 888 EMCCD, 1.2x auxiliary magnification
  • MetaMorph 7.8 system ID 8562