DeltaVision Elite Deconvolution Microscope

DeltaVision Elite Deconvolution Microscope

User Manual(s)

Location

FFSC 4228

Capabilities

  • Widefield imaging of living and fixed samples
  • Optimized deconvolution
  • Solid-state illumination, fast excitation switching
  • Temperature, humidity, CO2 control
  • Hardware-based autofocus
  • High-precision XYZ stage
  • Dual cameras - high-resolution and high-sensitivity
  • 405 nm and 488 nm lasers for photoactivation and photo-bleaching experiments
  • Optical axis integration/z-sweep acquisition
  • Kohler and critical illumination

Cost

$18.5/hr for first 3 hours, then $4/hr for the rest of imaging session

Detailed Specifications

The EM camera is currently removed from this system.

Link to API website

Microscope:

  • Olympus IX-71 base
  • API illuminator with Kohler and critical epi illumination
  • LWD DIC condensor with LED illumination
  • Ultimate focus - hardware based focus stability
  • High precision XYZ stage with inserts for slides and 35 mm dishes
  • Opaque Weather Station incubation system with regulated temperature, CO2 and humidity

InsightSSI 7 channel solid state illumination source with fast switch times:

  • Fiber coupled for homogenous scrambled light illumination
  • Monitor diode
  • DAPI 390/18
  • CFP 438/24
  • GFP/FITC 475/28
  • YFP 513/17
  • TRITC 542/27
  • mCherry/AF594 575/25
  • Cy5 632/22

Fluorescence attenuator:

  • Open (100% Transmission)
  • 50% T
  • 32% T
  • 10% T
  • 5% T
  • 2% T

Emission filterwheel 10 position:

  • Blank (for high speed)
  • CFP 470/24
  • YFP 559/38
  • mCherry/AF594 632/60
  • GFP/FITC 525/50
  • DAPI 435/48
  • TRITC 594/45
  • CY5 676/34
  • Analyzer (pol)

Eyepiece filter wheel 7 position (manual and encoded)

  • POL Analyzer
  • DAPI
  • TRITC
  • GFP/FITC
  • mCherry/AF594
  • CFP
  • YFP

Filter cubes/dichroics:

  1. Polychroic BGRD,FR (standard quad) DAPI,FITC,TRITC,CY5
  2. Polychroic BGmCherry,FR (alexaquad) DAPI,FITC,AF594,CY5
  3. CFP/YFP/mCherry
  4. GFP/mCherry (Chroma)
  5. Dual em pass CFP/YFP for high speed
  6. Dual em pass GFP/mcherry for high speed

Objectives:

  • 10x/0.40 UPlanSApo dry  WD 3.1 mm ∞/0.17/FN26.5, UIS2
  • 20x/0.75 UPlanSApo dry 1-U2B825 WD 0.65 mm DIC ∞/0.17/FN26.5, UIS2
  • 40x/0.65-1.35 oil UAPO40XOI3/340 1-UB768R WD 100 micron DIC ∞/0.17/FN22, UIS2, BFP1
  • 60x/1.42 oil PLAPON60XO WD 150 micron DIC ∞/0.17/FN26.5, UIS2, BFP1
  • 100x/1.40 oil UPLSAPO100X0 1-U2B836 WD 120 micron DIC ∞/0.17/FN26.5, UIS2

Stage

  • 50 mm travel in Y, 25 mm in X, 1 mm in Z

Cameras:

  • Coolsnap HQ2 high resolution CCD camera
  • Evolve back-thinned EM-CCD camera (512*512)

Lasers for photoactivation and photobleaching:

  • 488 nm 50 mW
  • 405 nm 50 mW

Computer:

  • Dual Xeon processors, 16 GB RAM, 2 TB RAID data array and 250 GB boot drive, 30" monitor
  • Linux (Centos 6.5) 64-bit
  • SoftWoRx 6.1, with system-level queuing
  • Similar system for offline deconvolution

This system was purchased with funding from the NIH/NCRR Shared Instrumentation Grant 1S10RR027528-01