DeltaVision Elite Deconvolution Microscope

Location
FFSC 4228Capabilities
- Widefield imaging of living and fixed samples
- Optimized deconvolution
- Solid-state illumination, fast excitation switching
- Temperature, humidity, CO2 control
- Hardware-based autofocus
- High-precision XYZ stage
- Dual cameras - high-resolution and high-sensitivity
- 405 nm and 488 nm lasers for photoactivation and photo-bleaching experiments
- Optical axis integration/z-sweep acquisition
- Kohler and critical illumination
Cost
$17 per hour for first 3 hours, then $3 per hourDetailed Specifications
The EM camera is currently removed from this system.
Microscope:
- Olympus IX-71 base
- API illuminator with Kohler and critical epi illumination
- LWD DIC condensor with LED illumination
- Ultimate focus - hardware based focus stability
- High precision XYZ stage with inserts for slides and 35 mm dishes
- Opaque Weather Station incubation system with regulated temperature, CO2 and humidity
InsightSSI 7 channel solid state illumination source with fast switch times:
- Fiber coupled for homogenous scrambled light illumination
- Monitor diode
- DAPI 390/18
- CFP 438/24
- GFP/FITC 475/28
- YFP 513/17
- TRITC 542/27
- mCherry/AF594 575/25
- Cy5 632/22
Fluorescence attenuator:
- Open (100% Transmission)
- 50% T
- 32% T
- 10% T
- 5% T
- 2% T
Emission filterwheel 10 position:
- Blank (for high speed)
- CFP 470/24
- YFP 559/38
- mCherry/AF594 632/60
- GFP/FITC 525/50
- DAPI 435/48
- TRITC 594/45
- CY5 676/34
- Analyzer (pol)
Eyepiece filter wheel 7 position (manual and encoded)
- POL Analyzer
- DAPI
- TRITC
- GFP/FITC
- mCherry/AF594
- CFP
- YFP
Filter cubes/dichroics:
- Polychroic BGRD,FR (standard quad) DAPI,FITC,TRITC,CY5
- Polychroic BGmCherry,FR (alexaquad) DAPI,FITC,AF594,CY5
- CFP/YFP/mCherry
- GFP/mCherry (Chroma)
- Dual em pass CFP/YFP for high speed
- Dual em pass GFP/mcherry for high speed
Objectives:
- 10x/0.40 UPlanSApo dry WD 3.1 mm ∞/0.17/FN26.5, UIS2
- 20x/0.75 UPlanSApo dry 1-U2B825 WD 0.65 mm DIC ∞/0.17/FN26.5, UIS2
- 40x/0.65-1.35 oil UAPO40XOI3/340 1-UB768R WD 100 micron DIC ∞/0.17/FN22, UIS2, BFP1
- 60x/1.42 oil PLAPON60XO WD 150 micron DIC ∞/0.17/FN26.5, UIS2, BFP1
- 100x/1.40 oil UPLSAPO100X0 1-U2B836 WD 120 micron DIC ∞/0.17/FN26.5, UIS2
Stage
- 50 mm travel in Y, 25 mm in X, 1 mm in Z
Cameras:
- Coolsnap HQ2 high resolution CCD camera
- Evolve back-thinned EM-CCD camera (512*512)
Lasers for photoactivation and photobleaching:
- 488 nm 50 mW
- 405 nm 50 mW
Computer:
- Dual Xeon processors, 16 GB RAM, 2 TB RAID data array and 250 GB boot drive, 30" monitor
- Linux (Centos 6.5) 64-bit
- SoftWoRx 6.1, with system-level queuing
- Similar system for offline deconvolution
This system was purchased with funding from the NIH/NCRR Shared Instrumentation Grant 1S10RR027528-01