Live Cell Station 1: Zeiss Axio Observer Z1

Location
LSRC B362E (entering through B359)Capabilities
- Imaging living samples and fixed samples - any fluorescent protein
- Temperature, humidity, CO2 control
- High sensitivity - EMCCD camera
- Multiposition timelapse
- Tiled images
Cost
$17 per hour for first 3 hours, then $3 per hourDetailed Specifications
Detailed specifications:
Zeiss Axio Observer Z1 motorized
Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and multiwell plates.
Pecon XL S1 incubator and control modules
X-CITE 120XL metal halide fluorescence light source
Filter cubes:
- Analyzer
- DAPI filter cube 49 (BP G365, FT 395, BP 445/50)
- Cy5 filter cube (Semrock FF01-630/38, FF655-Di01, FF01-694/44)
- GFP filter cube HE38 (BP 470/40, FT 495, BP 525/50)
- YFP filter cube HE46 (BP 500/20, FT 515, BP 535/30)
- Red filter cube HQ45 (BP 560/40, FT 585, BP 630/75)
FRET cube available (Semrock x438/24, FF458-Di01, m542/27) - swap into position 4 for ratiometric CFP/YFP FRET
Also available:
- CFP filter cube HE47 (BP 436/25, FT 455, BP 480/40)
- Cy7 filter cube (BP710/75, FT750, BP810/90)
Fluorescence attenuator:
- Open (100% Transmission)
- 70% T
- 50% T
- 40% T
- 20% T
- 2% T
Objectives:
- 2.5x/0.075 EC-Plan-NeoFluar (440310-9903) WD: 9.5mm
- 5x/0.16 Plan-NeoFluar (440320-9902) WD: 18.5mm
- 10x/0.30 Plan-NeoFluar Ph1, (440331-9902) WD: 5.2mm
- 20x/0.80 Plan-Apo DICII, (440640-9903) WD: 0.55mm, Coverglass: 0.17mm
- 40x/0.75 EC-Plan-NeoFluar DICII, (440350-9903) WD 0.71mm Thread type: M0.8X1/36”
- 100x/1.46 (Oil) Plan Apochromat DICIII (420792-9800) WD: 0.11mm, Coverglass: 0.17mm, (UV)VIS-IR
- Available: 40x/1.3 oil Plan-NeoFluar DICII (440452-9903) WD: 0.20mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
- Available: 63x/1.4 oil Plan Apochromat DICII (440762-9904) WD: 0.19mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
Calibrations for Photometrics Evolve EMCCD and CoolSNAP ES2 cameras:
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2.5x EM px=6.1650 um, CoolSNAP px=2.5290 um
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5x EM px= 3.0828 um, CoolSNAP px= 1.2645 um
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10x EM px= 1.5556 um, CoolSNAP px= 0.6135 um
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20x EM px= 0.7726 um, CoolSNAP px= 0.3091 um
-
40x EM px= 0.3810 um, CoolSNAP px= 0.1570 um
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63x EM px= 0.2423 um, CoolSNAP px= 0.0983 um
-
100x EM px= 0.1558 um, CoolSNAP px=0.0629 um
Cameras:
- Photometrics Evolve back-thinned EM-CCD camera (512*512)
- Photometrics CoolSNAP ES2 CCD camera (1392*1040)
Computer:
- HP Z4 G4 Worstation, Intel W-2133 3.6GHz processor, 32GB RAM, Nvidia Quadro P620 2GB graphics
- Windows 10 64-bit
- MetaMorph 7.10.1, system ID 32330
- ZEN Pro 2.3