Leica Stellaris 8 and FLIM

Stellaris8

User Manual(s)

Location

LSRC C142

Capabilities

  • Leica point scanning confocal microscope
  • Inverted Leica DMI8 microscope stand with Adaptive Focus Control and x,y scanning stage
  • Pulsed White Light Laser (440nm-790nm) for imaging wide-range of fluorophores from blue to near infrared
  • 5 high-sensitivity and low-noise spectral HyD detectors, all with photon counting and time-gating capability
  • Imaging based on spectrum or by fluorescence lifetime: TauSense and Fluorescent Lifetime Imaging Microscopy (FLIM).
  • Notch filters to further reduce background
  • Conventional and resonant scanners
  • LAS X software with 'LAS X 3D' image viewer and 'LAS X Navigator' for convenient tile-scanning and stitching

Cost

$30 per hour

Priority User Statement

This Leica Stellaris 8 confocal system was obtained with an NIH S10 grant award 1S10-OD034340-01A1. Any presentation or publication must site this NIH grant as per NIH requirements. Thank you.

Detailed Specifications

Microscope: 

  • Leica DMI8 inverted microscope stand for imaging fixed or live samples
  • LED light modules for observation under eyepieces
  • Motorized scanning stage with adapter for slides, chambered coverslips, single 35mm or 60mm dishes, multiwell plates, etc.

Conventional fluorescence filters and LED light sources for observation under eyepieces:

  • blue (LED 405)
  • green (GFP)
  • red (Rhod)
  • cyan and yellow (CFP/YFP)

Scanners:

  • Conventional scanner (up to 600Hz without zooming in)
  • Resonant scanner (8000Hz)

Lasers:

  • 405nm continuous wave laser
  • White Light Laser (pulse laser at 80MHz, 440nm-790nm range, tunable at 1nm steps)

Objective Lenses:

  • 10x/0.40 HC PL APO CS2, dry, working distance: 2.56 mm
  • 20x/0.75 HC PL APO CS2, dry, working distance: 0.62mm
  • 40x/1.30 Oil HC PL APO CS2, Oil immersion. working distance: 0.17 mm
  • 63x/1.40 Oil HC PL APO CS2, Oil immersion. working distance: 0.14 mm
  • 20x/0.75 IMM CORR HC PL APO CS2, compatible with oil, water, glycerine and silicon oil immersions with correction collar, working distance: 0.66 mm (water immersion and 0.17 mm coverglass).

Dichroic:

  • AOBS serves as tunable dichroic for all lasers

Detectors:

  • Total of 5 spectral detectors with emission band freely tunable at 1nm resolution
  • All have photon-counting and time-gating capability
  • Power HyD S detectors at positions 1, 3, and 5
  • Power HyD X detectors at positions 2, and 4, specialized for FALCON (FAst Lifetime CONtrast)

Programmable Control Panel

Computer:

  • HP Z6, Windows 10 IOT Enterprise LTSC, 64-bit
  • Intel Xeon W5-3425 3.19 GHz CPU, 128 GB memory
  • Display card: NVIDIA RTX A5000, 24GB GPU MEMORY
  • Data drive: 4TB Western Digital SSD PCIe Gen4 NVMe SSD Drive

Software: 

  • Leica LAS X with 2D analysis
  • LAS X Navigator
  • LAS X Dye Finder
  • LAS X Assay Editor
  • LAS X MicroLab
  • LAS X 3D Viewer