Olympus VivaView FL Incubator Microscope

Olympus VivaView FL Incubator Microscope

User Manual(s)

Location

MSRB3 - 4221

Capabilities

  • Incubation system allowing multi-day timelapse
  • Fluorescence and transmitted light DIC imaging
  • Up to 8 samples can be imaged per timelapse
  • Multiposition timelapse
  • Z-stacks
  • Liquid handling - change media or add drugs

Cost

$18.5/hr for first 3 hours, then $4/hr for the rest of imaging session

Detailed Specifications

Link to vendor website

Imaging modes:

  • DIC and brightfield transmitted light imaging
  • Fluorescence - CFP, GFP, YFP, Red

Sample formats:

Objectives:

  • 20x/0.75 DIC Olympus UPlanSAPO, WD: 0.65mm
  • (40x/0.95 DIC UPlanSApo, WD: 0.18mm objective is available, ask Gao)
  • (currently unavailable: 0.5x and 2.0x auxilary magnification available to produce 10x and 80x magnification/fov)

Pixel sizes:

  • 20x=0.32 micron
  • 40x=0.16 micron

Filters:

  • CFP - 436/20x 455 480/40m
  • GFP - 460-480HQ 485 495-540HQ
  • YFP - 500/20x 515 535/30m
  • Red - 535-555HQ 565 570-625HQ

Camera and Computer:

  • High performance workstation (Windows 10 Professional, 64-bit, Intel Xeon ES-1620 V3 @3.5GHz processor, 32GB memory)
  • Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera
  • MetaMorph premier acquisition (System ID 33991)

This system was initially purchased with funding from the North Carolina Biotechnology Center (IDG #2011-IDG-1002)