Zeiss ELRYA7 super-resolution

Zeiss ELYRA7

Location

Sands 415

Capabilities

The ZEISS Elyra 7 is a versatile super-resolution microscope system which combines high-speed live-cell imaging with subcellular detail, offering resolution of 100-120nm with SIM and even down to 60 nm with Lattice SIM² or 20-30 nm with SMLM - single molecule localization microscopy (like PALM/dSTORM) for molecular precision. Its key capabilities include fast 3D imaging with dual sCMOS cameras, gentle illumination for live samples, wide-field capabilities, DIC, and TIRF or HILO.

Highly inclined and laminated optical sheet (HILO) and total internal reflection illumination (TIRF).

Apotome 2: Grid-based optical sectioning to create highly contrasted images with high lateral and axial resolution. 

Cost

$42 per hour

Priority User Statement

This Zeiss ELYRA7 system was purchased with an NIH S10 shared instrumentation grant. Please include the following grant number in any publications or presentations: 1 S10 OD028703-01

Detailed Specifications

Lasers:

  • 405 nm (100 mw)
  • 488 nm (500 mW)
  • 561 nm (500 mW)
  • 637 nm (500 mW) 

Camera:  PCO.edge 4.2 (two with DualCam connector)

Filters / dichroics

  • LP560 - between cameras
  • BP 420-480 + BP 570-620
  •  

Microscope:

  • Zeiss Axio Observer 7
  • Märzhäuser motorized stage with linear encoding
  • 500 micron piezo for rapid z-control
  • Pecon dark enclosure chamber with heat and CO2 control

Objectives:

  • Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA (for SIM)
  • 63x/1.46 alpha Plan-Apochromat Corr, Oil, (for TIRF or SMLM)
  • 40x/1.4 Plan-APO Oil DIC (UV) VIS-IR
  • 20x/0.8 Plan-APO Dry
  • 0x/0.30 EC Plan-Neofluar Dry WD=5.2

Zoom Optics (for both cameras):

  • 1X
  • 1.6X

Computer workstations:

  • SIM processing and SMLM processing workstation
  • Storage workstation with 36TB hard drive; directly connected via 10Gigabit cable to the acquisition workstation
  • Acquisition workstation