Zeiss 880 Airyscan Inverted Confocal - FFSC

Capabilities

• High sensitivity point scanning confocal imaging
• Spectral array GaAsP detectors
• AiryScan module with up to 2x resolution gain and high SNR (pdf)
• Fixed and live samples
• FCS and FCCS

Detailed Specifications

See: The Basic Principle of Airyscanning (pdf).

Microscope:

• Zeiss Axio Observer Z1 with Definite Focus2
• Motorized XY stage
• Metal halide fluorescence source for widefield eyepiece visualization
• Dapi, Green, Red fluorescence cubes

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 477, 488, 514nm)
• 561 nm Diode laser
• HeNe 633nm

Objectives:

• 10x/0.30 (420340-9901): EC Plan-Neofluar, NA: 0.3, air, WD: 5.2mm
• 20x/0.80 (420650-9901): Plan-Apochromat, NA: 0.80, air, WD: 0.55mm
• 40x/1.2 (421767-9971): C-Apochromat, NA: 1.2, Water, Corr (coverglass: 0.14-0.19mm), FCS, WD:0.28mm
• 40x/1.3 (420462-9900): EC Plan-Neofluar, NA: 1.3, oil, WD: 0.21mm
• 63x/1.4 (420782-9900): Plan-Apochromat, NA: 1.4, Oil, WD: 0.19mm

Detectors:

• 34 channel confocal detector array for versatilely
• GaAsP high QE 32 channel spectral array with unmixing capability
• 2 standard alkali PMTs in positions for blue and far-red detection
• Photon counting possible with GaAsP array and PMT2 (cooled)
• AiryScan detector module and AiryScan Fast capability
• Transmitted light detector

Computer:

• HP workstation Z640
• Windows 7 Professional, 64bit
• CPU: Intel Xeon ES-2623 v3 3.0 GHz; Memory: 32GB
• Zeiss Zen 2.3
• Direct network link to FFSC-64 bit analysis workstation