Zeiss 780 Upright Confocal

User Manual(s)

• Zeiss 780 upright confocal manual

Capabilities

• High sensitivity point scanning confocal imaging
• Spectral array GaAsP detector
• Upright motorized stage microscope
• In vivo or in vitro samples
• FCS

Detailed Specifications

Microscope:

• Zeiss Axio Examiner with fixed stage (mechanical)
• 4 Objective turret
• Metal halide fluorescence source for widefield eyepiece visualization
• DAPI, Green, Red fluorescence cubes

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 488, and 514nm lines available)
• 561 nm Diode laser
• HeNe 633nm

Objectives:

• 2.5x/0.075 Dry EC Plan NeoFluar 440310-9903 WD 9.5 mm (normally in the drawer)
• 5x/0.15 Dry Plan NeoFluar 440320 WD 18.5 mm (normally in the drawer)
• 10x/0.45 Dry Zeiss Plan-Apochromat 1063-139 WD 2.0 mm
• 20x/0.80 Dry Zeiss Plan-Apochromat 440640-9903 WD 0.55 mm
• 20x/1.0 Water Zeiss W Plan-Apochromat 421452-9800 WD 1.8 mm
• 63x/1.4 Oil Zeiss Plan-Apochromat 44 07 62 (02) WD 0.19 mm

Dichroic(s):

• Twin gate dichroic system
• Low angle of incidence for improved reflection suppression

Detectors:

• 34 channel confocal detector array for versatilely
• GaAsP high QE 32 channel spectral array with unmixing capability
• 2 standard alkali PMTs in positions for blue and far-red detection
• Photon counting possible with GaAsP array and PMT2 (cooled)
• Transmitted light detector

Computer:

• HP Z840 with Intel Xeon E5-2623 V3 3.00 Ghz processor, 16 GB RAM, 2TB data drive, 27" monitor
• Windows 10 Enterprise (2015 LTSB) 64-bit
• Zen 2.3 Black