Zeiss 780 Inverted Confocal

Zeiss 780 Inverted Confocal

User Manual(s)

Location

Nanaline 378A

Capabilities

  • High sensitivity point scanning confocal imaging
  • Spectral array GaAsP detectors
  • Inverted microscope
  • Incubation for live cell timelapse - temp and CO2 control
  • Definite focus for timelapse

Cost

$30 per hour

Detailed Specifications

Microscope:

  • Zeiss Axio Observer Z1
  • Motorized XY stage
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm

Objectives:

  • 10X/0.3 NA Dry Plan-Neofluar (420340-9901)
  • 20X/0.8 NA Dry Plan-Apochromat (420650-9901)
  • 40X/1.4 NA Oil Plan-Apochromat DIC, (UV) VIS-IR (420762-9900)
  • 63X/1.4 NA Oil Plan-Apochromat DIC (420782-9900)

Dichroic(s):

  • Twin gate dichroic system
  • Low angle of incidence for improved reflection suppression

 

Spectral array GaAsP Detectors

 

Detectors:

  • 34 channel confocal detector array for versatilely
  • GaAsP high QE 32 channel spectral array with unmixing capability
  • 2 standard alkali PMTs in positions for blue and far-red detection
  • Photon counting possible with GaAsP array and PMT2 (cooled)
  • Transmitted light detector

Computer:

  • X5650 2.67 GHz w/ 6GB RAM, 230 GB Boot, 2 TB Data
  • Windows 7 Ultimate 64bit
  • Zen 2011