Leica STED and confocal

Capabilities

• Stimulated Emission Depletion super-resolution imaging
• High sensitivity point scanning confocal with pulsed white light laser
• High-sensitivity GaAsP HyD detectors with gating and photon counting
• Spectrally tunable emission bands
• Resonant scanner for low photo-toxicity
• Inverted configuration
• 3D z-stacks, timelapse, stitching, multi-position timelapse

Detailed Specifications

Microscope:

• Leica DMi8 motorized inverted
• Motorized scanning stage

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 488, 514nm)
• White Light Laser (pulsed) - 470nm-670nm
• STED depletion lasers: 592nm, 660nm, 775nm

Tandem scanners (select at software initialization)

• Conventional scanner
• Resonant scanner (8 000 Hz) - 28 fps at 512*512

Conventional fluorescence filters for eyepiece vizualization:

• Blue (A = x340-380, dichroic 400, LP425)
• Green (I3 = xBP470-490, dichroic 510, mLP515)
• Red (N2.1 = x515-560, dichroic 580, mLP590

Objectives:

• 10x NA 0.4 HC PL APO dry WD 2.2 mm
• 20x NA 0.75 HC PL APO multi-Imm Corr (oil, water, glycerol) DIC WD 270 um
• 40x NA 1.3 oil HC PL APO CS2 OIL DIC WD 240 um
• 100x/1.4 HCX PL APO OIL DIC WD 90 um
• 93x NA 1.3 glycerol with motorized correction collar (for STED) WD 0.3mm

Detectors:

• Five channels - two PMTs, three HyDs - high sensitivity Hybrid Detectors - PMT1 | HyD2 | PMT3 | HyD4 | HyD5
• All spectral detectors; HyDs have gating for STED

Computer:

• HP Z840 Workstation
• Windows 7
• LAS X
• Huygens deconvolution software linked to LAS X for STED deconvolution