Leica SP8 Upright Confocal

Capabilities

• High sensitivity point scanning confocal
• High-sensitivity GaAsP HyD detectors with photon counting
• Spectrally tunable emission band ranges
• Resonant scanner for low photo-toxicity
• Upright configuration
• 3D z-stacks, timelapse, stitching, multi-position timelapse

Detailed Specifications

Microscope:

• Leica DM6000CS stand (upright)
• Motorized scanning stage

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 488, 514nm)
• 561 nm Diode laser
• HeNe 633nm

Tandem scanners (select at software initialization)

• Conventional scanner
• Resonant scanner (8 000 Hz) - 28 fps at 512*512

Conventional fluorescence filters for eyepiece vizualization:

• Blue (A = x340-380, dichroic 400, LP425)
• Green (I3 = xBP470-490, dichroic 510, mLP515)
• Red (N2.1 = x515-560, dichroic 580, mLP590

Objectives:

• 2.5x/0.07 N PLAN WD 11.2 mm
• 5x/0.15 HCX PL FLUOTAR WD 13.7 mm
• 10x/0.3 HC PL FLUOTAR WD 11 mm
• 20x/0.75 HC PL APO 20x CS2 dry WD 0.62mm
• 25x/0.95 HCXIRAPO WATER dipping lens 2.4 mm DIC
• 40x/1.3 HC Plan APO CS2 OIL DIC WD 240 um
• 100x/1.4 HCX PL APO OIL DIC WD 90 um

Dichroic(s):

• Low incidence angle dichroics with notch filters to suppress background

Detectors:

• Four channels - two PMTs, two HyDs - high sensitivity Hybrid Detectors - HyD1 | PMT2 | HyD3 | PMT4
• All spectral detectors
• Motorized transmitted light detector

Computer:

• HP Z6 Workstation
• Windows 10
• LAS AF 3