Leica SP8 2photon DIVE

Capabilities

• Multiphoton imaging of thick in vivo and in vitro samples
• Long working distance objectives optimized for multiphoton
• Coherent Vision II femptosecond Ti:Saph laser (680-1080 nm) with pulse compensation
• SpectraPhysics Insight 3x tunable laser (680-1300 nm) with pulse compensation
• 4 HyD (sensitive) adjustable wavelength non-descanned detectors
• Tiled z-stack acquisition
• Scientifica animal stage; can also hold slides and dishes

Detailed Specifications

This system is the newest multi-photon system on the market with wavelength adjustable detection ranges on all four sensitive HyD detectors. One confocal path PMT also available.

Lasers:

• Chameleon Vision II single box Ti:Saph fsec laser with pulse compensation;
  • Tunable range 680-1080 nm @40 nm/sec, 80 MHz rep rate, 140 fsec pulse width
• Spectra Physics Insight 3x with 680-1300nm tunable range

Two photon laser and wavelength information:

Link to Two-photon cross sections at Cornell | MPE spectra from Olympus

Excitation beam optics:

• Intensity control via AOM

Microscope:

• Leica DM6 upright microscope stand
• Scientifica XY motorized stage with 2 exchangeable platforms
• 25x 1.0 NA HC IRAPO L  W motCORR (motorized correction) water dipping objective WD 2.6 mm
• 16x 0.6 NA HC FLUOTAR L VISIR with correction collar WD 2.5mm

Detectors:

• 4 sensitive HyD non-descanned detectors in the epi position
• Adjustable emission bands available controlled in software