Leica SP8 2photon DIVE

Location

RPII 112

Capabilities

  • Multiphoton imaging of thick in vivo and in vitro samples
  • Long working distance objectives optimized for multiphoton
  • Tunable fSec Ti:Saph laser (680-1080 nm) with pulse compensation
  • Tunable fSec Ti:Saph laser (680-1300 nm) wiht pulse compensation
  • 4 HyD (sensitive) non-descanned detectors
  • Tiled z-stack acquisition
  • Scientifica animal stage; also slides and dishes

Cost

$45.50 per hour base rate, discounted for bulk use

Detailed Specifications

This system is the newest multi-photon system on the market with wavelength adjustable detection ranges on all four sensitive HyD detectors. One confocal path PMT also available.

Lasers:

Excitation beam optics:

  • Intensity control via AOM

Microscope:

  • Leica DM6 upright
  • Scientifica XY motorized stage with 2 exchangeable platforms
  • 25x/1.05 NA water motorized correction objective WD 1.8 mm

Detectors:

  • 4 sensitive HyD non-descanned detectors in the epi position
  • Adjustable emission bands available controlled in software

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