Zeiss Axio Observer Widefield Fluorescence Microscope

Location
FFSC 4226Capabilities
- Multi-channel fluorescence imaging - DAPI, CFP, green, YFP, red and far-red
- Phase contrast and DIC optics
- Ideal for thin fixed specimens (oil objectives)
- Multi-well plate holder for TC cell documentation
Cost
$15 per hourDetailed Specifications
Microscope:
- Zeiss Axio Observer A1 inverted stand
- Manual stage with insert for slides, dish and multi-well plates
- Zeiss HBO arc lamp and power supply
Filter cubes:
- DIC
- DAPI (FS49: G365, FT395, BP445/50)
- CY5 (filter set 50 BP640/30, FT660, BP690/50)
- Green (FS38HE: BP470/40, FT495, BP525/50)
- YFP (FS46: BP500/20, FT515, BP535/30)
- Red (FS43HE: BP550/25, FT570, BP605/70)
- (available) CFP (FS47: BP436/20, FT455, BP480/40)
- (available) CY7 (BP710/75, FT750, BP810/90)
Manual 6-position fluorescence attenuator : 100, 70, 50, 40, 20 and
Objectives:
- 5x/0.16 Ph1 440321-9902: EC Plan-Neofluar, NA: 0.16, air, Ph1, WD: 18.5mm
- 10x/0.3 Ph1 440331-9902: EC Plan-Neofluar, NA: 0.3, air, Ph1, WD: 5.2mm
- 20x/0.5 Ph2 440341-03: EC Plan-Neofluar, NA: 0.50, Ph2, WD: 2.0mm, coverglass: 0.17mm
- 40x/0.75 Ph2 440351-9903: EC Plan-Neofluar, NA: 0.75, air, Ph2, WD: 0.71mm, coverglass: 0.17mm
- 63x/1.40 DICIII 420760-9905: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.19mm, coverglass: 0.17mm, Thread type: M0.8X1/36"
- 100x/1.4 Ph3 440781-02: Plan-Apochromat, NA: 1.40, oil, Ph3, WD: 0.17mm, coverglass: 0.17mm
Scale (micron/pixel):
- 5x/0.16 px = 1.262 micron
- 10x/0.3 px = 0.627micron
- 20x/0.5 px = 0.311 micron
- 40x/0.75 px = 0.156micron
- 63x/1.40 px = 0.100 micron
- 100x/1.4 px = 0.063 micron
Camera: Hamamatsu Orca-R2 (C10600-10B) digital camera (1344 x 1024) & controller
Computer:
- Dell Precision T7600, Intel Xeon E5-2630, 2.3GHz, 12GB RAM, Windows 10 Enterprise, 64-bit
- MetaMorph 7.8.13, system ID 3502