Zeiss Axio Observer Widefield Fluorescence Microscope

User Manual(s)

• Zeiss Axio Observer Manual

Capabilities

• Multi-channel fluorescence imaging - DAPI, CFP, green, YFP, red and far-red
• Phase contrast and DIC optics
• Ideal for thin fixed specimens (oil objectives)
• Multi-well plate holder for TC cell documentation

Detailed Specifications

Microscope:

• Zeiss Axio Observer A1 inverted stand
• Manual stage with insert for slides, dish and multi-well plates
• Zeiss HBO arc lamp and power supply

Filter cubes:

1. DIC
2. DAPI (Zeiss Filter Set 49: G365, FT395, BP445/50)
3. Cy5 (Zeiss Filter Set 50: BP640/30, FT660, BP690/50)
4. Green (Zeiss Filter Set 38HE: BP470/40, FT495, BP525/50)
5. YFP (Zeiss Filter Set 46: BP500/25, FT515, BP535/30)
6. Red (Zeiss Filter Set 43HE: BP550/25, FT570, BP605/70)
7. (available) CFP (Zeiss Filter Set 47: BP436/20, FT455, BP480/40)
8. (available) CY7 (BP710/75, FT750, BP810/90)

Manual 6-position fluorescence attenuator (%) : 100, 70, 50, 40, and 20

Objectives:

• 5x/0.16 Ph1 440321-9902: EC Plan-Neofluar, NA: 0.16, air, Ph1, WD: 18.5mm
• 10x/0.3 Ph1 440331-9902: EC Plan-Neofluar, NA: 0.3, air, Ph1, WD: 5.2mm
• 20x/0.5 Ph2 440341-03: EC Plan-Neofluar, NA: 0.50, Ph2, WD: 2.0mm, coverglass: 0.17mm
• 40x/0.75 Ph2 440351-9903: EC Plan-Neofluar, NA: 0.75, air, Ph2, WD: 0.71mm, coverglass: 0.17mm
• 63x/1.40 DICIII 420760-9905: Plan-Apochromat, NA: 1.40, oil, DIC, WD: 0.19mm, coverglass: 0.17mm, Thread type: M0.8X1/36"
• 100x/1.4 Ph3 440781-02: Plan-Apochromat, NA: 1.40, oil, Ph3, WD: 0.17mm, coverglass: 0.17mm

Scale (micron/pixel):

• 5x/0.16  px = 1.262 micron
• 10x/0.3  px = 0.627micron
• 20x/0.5  px = 0.311 micron
• 40x/0.75  px = 0.156micron
• 63x/1.40  px = 0.100 micron
• 100x/1.4  px = 0.063 micron

Camera: Hamamatsu Orca-R2 (C10600-10B) digital camera (1344 x 1024) & controller

Computer:

• Dell Precision T7600, Intel Xeon E5-2630, 2.3GHz, 12GB RAM, Windows 10 Enterprise, 64-bit
• MetaMorph 7.8.13, system ID 3502