Zeiss Axio Imager Widefield Fluorescence Microscope

Zeiss Axio Imager Widefield Fluorescence Microscope

User Manual(s)

Location

LSRC C171

Capabilities

  • Widefield fluorescence imaging
  • Motorized stage for tiling/stitching
  • Transmitted light images - brightfield, DIC
  • Colour camera for IHC, H&E, etc. staining
  • Z-stacks
  • Apotome for optical sectioning
  • Polarization microscopy

Cost

$18 per hour

Detailed Specifications

Upright AxioImager.Z2 microscope stand

Motorized stage - 75 x 50

Apotome.2 - optical sectioning using structured illumination

HXP 120 metal halide fluorescence illumination with intensity control. LED transmitted light illumination.

Filter cube sets (10 position turret):

  1. Brightfield/DIC analyzer
  2. DAPI, Hoechst (Zeiss Filter Set 47, Chroma 31000v2 D350/50x, 400DCLP, D460-50)
  3. CFP (Zeiss Filter Set 47, Chroma 31044v2 D436/20, 455DCLP, D480/40)
  4. Green, GFP, FITC, Alexa488 (Zeiss Filter Set 38 HE, BP 470/40, FT 495, BP 525/50)
  5. YFP (Zeiss Filter Set 46, Chroma 41028 HQ500/25, Q515LP, HQ535/30)
  6. Rhodamine, DsRed, Alexa555, Alexa568 (Zeiss cube set 43 HE, BP 550/25, FT 570, BP 605/70)
  7. Texas Red, mCherry (Zeiss Filter Set 45, BP 560/40, FT 585, BP 630/75)
  8. Cy5, Alexa633, Alexa647 (Zeiss Filter Set 50, BP 640/30, FT 660, BP 690/50)
  9. Cy7 (BP 710/75, FT750, BP810/90)

Objectives:

  • 5x/0.16 Plan-Apochromat, NA: 0.16, air, WD: 12.1 mm
  • 10x/0.45 Plan-Apochromat, NA: 0.45, air, WD: 2.0 mm
  • 20x/0.80 DIC, Plan-Apochromat, NA: 0.80, air, WD: 0.55 mm, coverglass: 0.17 mm
  • 40x/0.75 DIC, EC Plan-NeoFluar, NA: 0.75, air, WD: 0.41 mm, coverglass: 0.17 mm
  • 63x/1.4 oil DIC (420782-9900), Plan-Apochromat, NA: 1.40, WD: 0.19 mm, coverglass: 0.17 mm
  • 100x/1.4 oil DIC (420780-02), Plan-Apochromat, NA: 1.40, WD: 0.17 mm, coverglass: 0.17 mm

Pixel sizes in microns (both cameras) :

  • 5x: 0.91um
  • 10x: 0.45um
  • 20x: 0.23um
  • 40x: 0.11um
  • 63x: 0.072um
  • 100x: 0.045um.

Cameras: Dual camera ports

  • Axiocam 506 color
  • Axiocam 506 mono

Computer:

  • Zeiss Imaging workstation Win10 Enterprise, 64 GB RAM
  • Zen 2.6 Pro with modules for z-stacks, time-lapse, tiling, autofocus.

This system was substantially upgraded in October 2015.