Capabilities

• Widefield fluorescence imaging
• Motorized stage for stitching/tiling
• Transmitted light images - brightfield, DIC
• Colour camera for IHC (e.g. H&E staining)
• Z-stacks
• Apotome for optical sectioning

Detailed Specifications

Upright AxioImager.Z2 microscope stand

Motorized stage - 75 x 50

Apotome.2 - optical sectioning using structured illumination

HXP 120 Metal halide fluorescence illumination with intensity control. LED transmitted light illumination.

Filter cube sets (10 position turret):

1. DIC analyzer
2. DAPI, Hoechst (Chroma 31000v2 D350/50x, 400DCLP, D460-50)
3. CFP (Chroma 31044v2 D436/20, 455DCLP, D480/40)
4. Green, GFP, FITC, Alexa488 (Zeiss Filter Set 38 HE 489038-9901-000, BP 470/40, FT 495, BP 525/50)
5. YFP (Chroma 41028 HQ500/20, Q515LP, HQ535/30)
6. Rhodamine (Zeiss cube set 43 HE, BP 550/25, FT 570, BP 605/70)
7. Texas Red (Chroma 41004 Alexa 568, Alexa 594 HQ560/55, Q595LP, HQ645/75)
8. CY5 Zeiss Filter Set 50 (488050-9901-000), BP 640/30, FT 660, BP 690/50
9. Brightfield (i.e. empty)

Objectives:

• 5x/0.16 Plan-Apochromat, NA: 0.16, air, WD: 12.1 mm, M27
• 10x/0.45 Plan-Apochromat, NA: 0.45, air, WD: 2.0 mm M27
• 20x/0.80 DIC :Plan-Apochromat, NA: 0.80, air, WD: 0.55 mm, coverglass: 0.17 mm M27
• 40x/0.9 DIC : EC Plan-NeoFluar, NA: 0.75, air, WD: 0.41 mm, coverglass: 0.17 mm M27
• 63x/1.4 oil DIC 420760-03: Plan-Apochromat, NA: 1.40, WD: 0.19 mm, coverglass: 0.17 mm, thread type: M27x0.75
• 100x/1.4 oil DIC 420780-02: Plan-Apochromat, NA: 1.40, WD: 0.17 mm, coverglass: 0.17 mm, thread type: M0.8X1/3

Pixel sizes in microns (both cameras) :

• 5x: 0.91um
• 10x: 0.45um
• 20x: 0.23um
• 40x: 0.11um
• 63x: 0.072um
• 100x: 0.045um.

Cameras: Dual camera ports

• Axiocam 506 color
• Axiocam 506 mono

Computer:

• Zeiss Imaging workstation Win7, 64 GB RAM
• Zen 2 with modules for z-stacks, Tiling, Autofocus.

This system was substantially upgraded in October 2015.