Zeiss Axio Imager Widefield Fluorescence Microscope

Location
LSRC C171Capabilities
- Widefield fluorescence imaging
- Motorized stage for stitching/tiling
- Transmitted light images - brightfield, DIC
- Colour camera for IHC (e.g. H&E staining)
- Z-stacks
- Apotome for optical sectioning
Cost
$15 per hourDetailed Specifications
Upright AxioImager.Z2 microscope stand
Motorized stage - 75 x 50
Apotome.2 - optical sectioning using structured illumination
HXP 120 Metal halide fluorescence illumination with intensity control. LED transmitted light illumination.
Filter cube sets (10 position turret):
- DIC analyzer
- DAPI, Hoechst (Chroma 31000v2 D350/50x, 400DCLP, D460-50)
- CFP (Chroma 31044v2 D436/20, 455DCLP, D480/40)
- Green, GFP, FITC, Alexa488 (Zeiss Filter Set 38 HE 489038-9901-000, BP 470/40, FT 495, BP 525/50)
- YFP (Chroma 41028 HQ500/20, Q515LP, HQ535/30)
- Rhodamine (Zeiss cube set 43 HE, BP 550/25, FT 570, BP 605/70)
- Texas Red (Chroma 41004 Alexa 568, Alexa 594 HQ560/55, Q595LP, HQ645/75)
- CY5 Zeiss Filter Set 50 (488050-9901-000), BP 640/30, FT 660, BP 690/50
- Brightfield (i.e. empty)
Objectives:
- 5x/0.16 Plan-Apochromat, NA: 0.16, air, WD: 12.1 mm, M27
- 10x/0.45 Plan-Apochromat, NA: 0.45, air, WD: 2.0 mm M27
- 20x/0.80 DIC :Plan-Apochromat, NA: 0.80, air, WD: 0.55 mm, coverglass: 0.17 mm M27
- 40x/0.9 DIC : EC Plan-NeoFluar, NA: 0.75, air, WD: 0.41 mm, coverglass: 0.17 mm M27
- 63x/1.4 oil DIC 420760-03: Plan-Apochromat, NA: 1.40, WD: 0.19 mm, coverglass: 0.17 mm, thread type: M27x0.75
- 100x/1.4 oil DIC 420780-02: Plan-Apochromat, NA: 1.40, WD: 0.17 mm, coverglass: 0.17 mm, thread type: M0.8X1/3
Pixel sizes in microns (both cameras) : 5x 0.91um, 10x 0.45um, 20x 0.23um, 40x 0.11um, 63x 0.072um, and 100x 0.045um.
Cameras: Dual camera ports
- Axiocam 506 color
- Axiocam 506 mono
Computer:
- Zeiss Imaging workstation Win7, 64 GB RAM
- Zen 2 with modules for z-stacks, Tiling, Autofocus.
This system was substantially upgraded in October 2015.