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Light Microscopy Core Facility

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collage of microscopes

 

The Light Microscopy Core Facility (LMCF) offers a wide range of confocal and conventional fluorescence microscopes and image analysis resources. As a centrally funded shared resource, the core’s aim is to offer affordable and efficient access to standard and advanced imaging instrumentation for users of all levels of experience and from any discipline across the Duke University and the Duke Medical Center campuses.

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News

  • ZeissElyra7
    Super-resolution available in the LMCF
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Featured Images

Dorsal closure in Drosophila.
Dorsal Closure in Drosophila
butterfly nuclei
Butterfly Nuclei
Sea Urchin Embryo Pharynx
Sea Urchin Embryo Pharynx
Podosomes
Podosomes
Liver Section
Liver Section
Skeletal Myoblasts
Skeletal Myoblasts

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Light Microscopy Core Facility (LMCF)

4215 French Family Science Center
124 Science Drive
Durham, NC 27708
lmcf@duke.edu

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Microscopes

Confocal Microscopes
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Widefield Fluorescence
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  • About LMCF
    • Personnel
    • Location
      • French Family Science Cluster
      • Levine Science Research Center Cluster
      • Medical Sciences Research Building III Cluster
      • Nanaline Duke Cluster
    • Governance
    • Publications
    • Information for Publications
    • Funding Acknowledgements
    • Gallery of Images
  • Microscopes & Equipment
    • Microscopes
      • Confocal Microscopes
      • Live Cell Imaging
      • Laser Capture Microdissection
      • Lightsheet Microscopes
      • Multiphoton Microscopes
      • Optical Trap
      • Stereoscopes & Dissecting
      • Super-resolution
      • TIRF
      • Widefield Fluorescence
    • Image Analysis Workstations & Software
  • Services
    • Links to Image Analysis Software
    • Equipment Manuals
      • Andor XD Spinning Disk Confocal Microscope Manual
      • Andor Dragonfly Spinning Disk Confocal Microscope (User's Manual)
      • DeltaVision Elite Deconvolution Microscope
      • Leica AM Total Internal Reflection Fluorescence Manual
      • Leica SP5 Inverted Confocal Manual
      • Live Cell Station (Zeiss Axio Observer Z1) Manual - With MetaMorph
      • Live Cell Station (Zeiss Axio Observer Z1) Manual - Single or Tile Imaging With ZEN
      • Olympus FV1000 Multiphoton Manual
      • Olympus VivaView FL Incubator Microscope Manual
      • ZEN 2.3 Pro: Single Field or Tiling/Stitching Multiple Fields
      • Zeiss 710 Inverted confocal manual
      • Zeiss 780 inverted confocal manual
      • Zeiss 780 upright confocal manual
      • Zeiss Axio Imager Z2 Upright Microscope Manual
      • Zeiss Axio Observer Manual
      • Zeiss LSM 510 Inverted Confocal Manual
      • Zeiss Lumar.V12 Stereoscope Manual
      • Zeiss MosaiX - Color brightfield tiling and image stitching on the LCM
      • Zeiss PALM MicroBeam Laser Capture Microdissection Manual
      • Zeiss 880 Inverted Confocal Manual
    • Guides & Tutorials
      • Introduction to Microscopy
      • Choose Best Microscope for your Sample
      • Introduction to Sample Preparation
        • Coverslip Thickness
        • Glass Coverslip Cleaning (Acid Wash)
        • Multiwell Chamber Slides
        • Paraformaldehyde, Formadehyde and Formalin
      • Safe Imaging of Live Samples
      • Laser Lines and Filters
      • Count Nuclear Foci - ImageJ
      • Overlay Images in ImageJ
      • Photoshop
      • Control Motorized Stage in MetaMorph
      • Kohler Illumination Procedure
      • Clean an Objective
      • File Formats for Microscopy
      • References
      • Imaris Learning Resources
    • Data Saving/Data Transfer
  • Booking
    • New Users
    • Request Training
    • Fees
    • Facility Rules
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