Olympus VivaView FL incubator microscope

Duke LMCF vivaview Capabilities:
  • Incubation system allowing multi-day timelapse
  • Fluorescence and transmitted light DIC imaging
  • Up to 8 samples can be imaged per timelapse
  • Multiposition timelapse
  • Z-stacks
  • Liquid handling - change media or add drugs

Location: Room 111 Research Park II

Cost: $14/hr for first 3 hours, then $2/hr base rate, discounted for bulk use

User manual: html

Request training | Reserve instrument

Detailed specifications

Link to vendor website

Imaging modes:

  • DIC and brightfield transmitted light imaging
  • Fluorescence - CFP, GFP, YFP, Red

Sample formats:

Objectives:

  • 20x/0.75 DIC Olympus UPlanSAPO 0.65 mm WD
  • 0.5x and 2.0x auxilary magnification available to produce 10x and 40x magnification/fov
  • (40x/0.95 DIC UPlanSApo, WD: 0.18mm objective is available, ask Sam or Gao)
  • Pixel sizes: 20x=0.32micron, 10x=0.64micron, 40x=0.16 micron

Filters:

  • CFP - 436/20x 455 480/40m
  • GFP - 460-480HQ 485 495-540HQ
  • YFP - 500/20x 515 535/30m
  • Red - 535-555 HQ 565 570-625HQ

Computer:

  • High performance workstation (Windows XP 32bit Xeon W5580)
  • MetaMorph premier acquisition (System ID 33991)

This system was purchased with funding from the North Carolina Biotechnology Center (IDG #2011-IDG-1002)