Leica AM TIRF MC

duke LMCF leica TIRF system Capabilities:
  • TIRF imaging
  • Automated alignment
  • Variable TIRF depth
  • 4 channels
  • EM-CCD camera
  • Environmental chamber

Location: Room C142 LSRC (613-8217)

Cost: $15 per hour

User manual: html | pdf

Request training | Reserve instrument

Detailed specifications:

Leica DMI6000 B motorized microscope

Environmental chamber, two-channel temperature control and CO2 regulation

Leica AM TIRF module - automatic alignment of TIRF illumination

Laser - 4 Diode lasers (405 488 561 635 nm) with AOTF control

EL-6000 metal halide fluorescence light source with discrete attentuator

Filter cubes:

  1. VBG triple
  2. 635 far-red
  3. Green only

Objectives:

  • 10x/0.40 HC PLAN APO
  • 20x/0.70 HC PLAN APO
  • 40x/0.75 HCX PLAN APO
  • 100x/1.46 oil TIRF objective PLAN APO DIC

Camera:

  • Hamamatsu imagEM backthinned EM-CCD camera (512*512), C9100-13

Computer:

  • Leica workstation, 2 GB RAM, Dual hard-drives
  • Windows XP Professional SP2
  • Leica LAS AF 1.8.0