Leica SP8 confocal microscope

Duke LMCF leica SP2 confocal Capabilities:
  • High sensitivity point scanning confocal
  • High-sensitivity GaAsP HyD detectors with photon counting
  • Spectrally tunable emission bands
  • Resonant scanner for low photo-toxicity
  • Upright configuration
  • 3D z-stacks, timelapse, stitching, multi-position timelapse

Location: Room 337C Nanaline Duke (681-4076)

Cost: $27 per hour base rate, discounted for bulk use

User manual:

Request training | Reserve instrument


Detailed specifications:

Microscope:

  • Leica DM6000CS stand (upright)
  • Motorized scanning stage

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm

Tandem scanners (select at software initialization)

  • Conventional scanner
  • Resonant scanner (8 000 Hz) - 28 fps at 512*512
Conventional fluorescence filters for eyepiece vizualization:
  • Blue (A = x340-380, dichroic 400, LP425)
  • Green (I3 = xBP470-490, dichroic 510, mLP515)
  • Red (N2.1 = x515-560, dichroic 580, mLP590
Objectives:
  • 2.5x/0.07 N PLAN WD 11.2 mm
  • 5x/0.15 HCX PL FLUOTAR WD 13.7 mm
  • 10x/0.3 HC PL FLUOTAR WD 11 mm
  • 20x/0.7 HC PL APO multi-Imm Corr (oil, water, glycerol) DIC WD 260 um
  • 25x/0.95 HCXIRAPO WATER dipping lens 2.4 mm DIC
  • 40x/1.3 HC Plan APO CS2 OIL DIC WD 240 um
  • 100x/1.4 HCX PL APO OIL DIC WD 90 um

Dichroic(s):

  • Low incidence angle dichroics with notch filters to supress background
Detectors:
  • Four channels - two PMTs, two HyDs - high sensitivity Hybrid Detectors - HyD1 | PMT2 | HyD3 | PMT4
  • All spectral detectors
  • Motorized transmitted light detector
Computer:
  • HP Z620 Workstation
  • Windows 7
  • LAS AF 3