Leica SP5 upright confocal microscope

Leica SP5 upright Duke LMCF Capabilities:
  • Imaging diverse fluorophores in fixed or live samples
  • Spectrally tunable detectors
  • Ultra-fast resonant scanning (reduced photo-damage with live samples)
  • 3D z-stacks, timelapse, multi-position timelapse

Location: 333D Nanaline Duke (inside 324/329)

Cost: $27 per hour base rate, discounted for bulk use

User manual:

Request training | Reserve instrument


This system was incorporated into LMCF to make it accesable to all at Duke. To ensure members of the Kuo, Bagnat and Eroglu labs have access to the machine they choose to share, they are able to book time up to 14 days ahead; other users can make reservations up to 7 days ahead.

Detailed specifications:

Microscope:

  • Leica DM6000CS
  • Metal halide fluorescence source
  • Motorized scanning stage

Tandem scanners (select at software initialization)

  • Conventional scanner
  • Resonant scanner (8 000 Hz)

Lasers:

  • 405 nm Diode laser
  • Argon (458, 488, 476nm, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm
Conventional fluorescence filters:
  • Analyzer
  • Dapi
  • Green
  • Red
Objectives:
  • 10x/0.40 10X HC PL APO CS, NA:0.40, air, WD: 2.2mm, coverglass: 0.17mm, M25
  • 20x/0.70 HC PL APO CS IMM, NA: 0.7, DIC, WD: 0.59mm, coverglass: 0.17mm, Thread type: M25.
  • 40x/1.25-0.75 oil : 40X, HCX PL APO, NA: 1.25, Oil, DIC, WD: 0.1mm, coverglass: 0.17mm, Iris diaphragm, Thread type: M25
  • 63x/1.4-0.60 oil : HCX PL APO, NA: 1.4, Oil, DIC, WD: 0.09mm, Coverglass: 0.17mm, Iris Diaphragm, Thread type: M25

Dichroic(s):

  • RT 30/70
  • Substrate
  • TD 488/561/633
  • DD 458/514
  • RSP 500
  • DD 488/561
Detectors:
  • Four channels
  • All cooled PMTs
  • All spectral detactors
  • Transmitted light detector
Computer:
  • HP xw8400 workstation with Intel Xeon 5160 CPU, 4 GB RAM, OS and data drives
  • Windows xp
  • Leica LAS AF 2.3