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Leica SP5 confocal microscope
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Capabilities:
- Imaging UV and VIS fluorophores in fixed or live
samples
- Spectral imaging
- Temperature, humidity, CO2 control
- Ultra-fast resonant scanning for live-cell
timelapse
- 3D z-stacks
- FRAP/FLIP/Photoactivation, FRET
Location:
C142 LSRC (613-8217)
Cost: $20 per hour
User manual: html |
pdf
Request
training | Reserve
instrument |
Detailed specifications:
Microscope:
- Leica DMI6000CS (inverted)
- Live cell chamber (Ludin cube)
- Metal halide fluorescence source
- Motorized scanning stage
Tandem scanners (select at software initialization)
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Conventional scanner
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Resonant scanner (8 000 Hz)
Lasers:
- 405 nm Diode laser
- Argon/2 (458, 477, 488, 496, 514nm)
- 561 nm Diode laser
- HeNe 594 nm
- HeNe 633nm
Conventional fluorescence filters:
Objectives:
- 10x/0.40 (Leica Plan Apochromat)
- 20x/0.70 (Leica Plan Apochromat)
- 40x/1.25-0.75 oil (Leica Plan NeoFluar)
- 63x/1.20 water (Leica Plan Apochromat)
- 100x/1.4-0.70 oil (Leica Plan Apochromat)
Dichroic(s):
- AOBS serves as tunable dichroic for all lasers
Detectors:
- Three channels
- All cooled PMTs
- All spectral detactors
- Transmitted light detector
Computer:
- HP xw6400 workstation Intel Xeon CPU, 4 GB RAM, Dual
hard-drives, CD/DVD burners
- MS Windows XP Professional SP2
- Leica LAS AF 1.8.2
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