Leica SP5 confocal microscope

Capabilities: Imaging diverse fluorophores in fixed or live samples Temperature, humidity, CO2 control Spectrally tunable detectors Ultra-fast resonant scanning (reduced photo-damage with live samples) 3D z-stacks, timelapse, multi-position timelapse FRAP/FLIP/Photoactivation, FRET (ratio or acceptor photobleaching) Location: C142 LSRC (613-8217) Cost: $25.75 per hour base rate, discounted for bulk use User manual: html | pdf Request training | Reserve instrument

Detailed specifications:

Microscope:

• Leica DMI6000CS (inverted)
• Live cell chamber (Ludin cube)
• Metal halide fluorescence source
• Motorized scanning stage

Tandem scanners (select at software initialization)

• Conventional scanner
• Resonant scanner (8 000 Hz)

Lasers:

• 405 nm Diode laser
• Argon/2 (458, 477, 488, 496, 514nm)
• 561 nm Diode laser
• HeNe 594 nm
• HeNe 633nm

• Analyzer
• Dapi
• Green
• Red

• 10x/0.40 11506284, 10X HC PL APO, NA:0.40, air, WD: 2.2mm, coverglass: 0.17mm, M25
• 20x/0.70 11506513: HC PL APO CS, NA: 0.7, air, DIC, WD: 0.59mm, coverglass: 0.17mm, Thread type: M25.
• 40x/1.25-0.75 oil 11506250: 40X, HCX PL APO, NA: 1.25, Oil, DIC, WD: 0.1mm, coverglass: 0.17mm, Iris diaphragm, Thread type: M25
• 63x/1.20 water 11506279: 63X, HCX PL APO W Corr CS, NA: 1.2, Water, DIC, WD: 0.22mm, Coverglass: 0.14mm-0.18mm, thread type: M25
• 100x/1.4-0.70 oil 11506210: HCX PL APO, NA: 1.4, Oil, DIC, WD: 0.09mm, Coverglass: 0.17mm, Iris Diaphragm, Thread type: M25

Dichroic(s):

• AOBS serves as tunable dichroic for all lasers

• Three channels
• All cooled PMTs
• All spectral detactors
• Transmitted light detector

• HP Z269 workstation with Intel Xeon E5-2650 CPU, 16 GB RAM, 2TB data drives
• Windows 7 64bit
• Leica LAS AF 2.6