Leica SP2 confocal microscope

The Leica SP2 has been replaced by an SP8

Duke LMCF leica SP2 confocal Capabilities:
  • Imaging diverse fluorophores in fixed or live samples
  • Temperature, humidity, CO2 control
  • 3D z-stacks, timelapse, stitching, multi-position timelapse

Location: Room 337C Nanaline Duke (681-4076)

Cost:


User manual:

Request training | Reserve instrument

Detailed specifications:

Microscope:

  • Leica DMIRE2 stand (inverted)
  • Live cell chamber
  • Motorized scanning stage
  • Z-galvo available

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 496, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm
Conventional fluorescence filters:
  • Analyzer
  • Green
  • Red
Objectives:
  • 10x/0.3 HC Plan Fluotar
  • 20x/0.7 HC Plan Apo
  • 20x/0.7 HC Plan Apo Imm Corr
  • 40x/1.25 HCX Plan Apo OIL
  • 63x/1.4 HCX Plan Apo OIL
  • 100x/1.4 HCX Plan Apo OIL

Dichroic(s):

  • AOBS serves as tunable dichroic for all lasers
Detectors:
  • Four channels
  • All spectral detectors
  • Transmitted light detector
Computer:
  • HP xw8200 workstation
  • Windows XP