Spinning disk confocal microscope 2

This system has permanently retired and this information is left here solely for reference. Many parts of the system were used for the axio observer

Duke LMCF Nicklas spinning disk confocal microscope Capabilities:
  • Imaging green and red fluorophores (eg GFP, Alexa 488/568)

Location: Room 4226 French Family Science Center (613-8097)

Cost: $15 per hour

Detailed specifications

Scope stand:
  • Zeiss Axiovert 100 TV
Scan head:
  • Yokogawa model CSU 10B-F300-E with slider filters
Excitation source:
  • Carl Zeiss AttoArc variable intensity lamp control
  • Laser Physics - 488 & 568 lines
Objectives:
  • 20x/0.5 Ph2 Plan Neofluar (44 03 41)
  • 40x/1.3 oil Ph3 Plan-Neofluar (44 04 51)
Princeton Instruments piezo stepper:
  • Hamamatsu Orca ER digital camera & controller
Shutters:
  • 2 Uniblitz model D122 (one fluor, one Phase/DIC)
Computer:
  • Dell precision 490 Dual core Xeon processor, 4GB RAM, Dual hard-drives, XP professional SP2
  • MetaMorph 7.5 system ID 3502