Andor XD revolution spinning disk confocal microscope

Installed and ready for use

LMCF Andor olympus revolution XD spinning disk confocal Capabilities:
  • High-speed multidimensional imaging with optical sectioning
  • Spinning disk modality produces high SNR images with minimal photobleaching
  • High sensitivity EMCCD camera
  • Laser lines for all classes of fluorscent proteins
  • Incubation for mammalian cells
  • IR reflection based autofocus
  • Silicon oil 60x objective for RI match

Location: Room C142 LSRC

Cost: $17.50 per hour base rate, discounted for bulk use

User manual:

Request training | Reserve instrument

Detailed specifications

Link to the Olympus website

Scanhead

  • Yokogawa CsuX-1 5000 rpm
  • Andor precision control and laser synchronization
  • Motorized dichroics - BGR and CYR

Lasers:

  • Andor AOTF control
  • 405 nm (100 mw) diode
  • 445 nm (40 mW) diode
  • 488 nm (50 mW) diode
  • 515 nm (50 mW) diode
  • 561 nm (50 mW) diode

Microscope:

  • IX81 motorized stand
  • ASI Motorized stage with linear encoding
  • 300 micron piezo for rapid z-control
  • Zero-drift compensation reflection based autofocus
  • BGR widefield fluorescence with metal halide source
  • Tokai Hit stage top incubation with CO2

Objectives:

  • 10x/0.3 UPlanFl dry
  • 20x/0.5 UplanFl dry
  • 40x/1.3 UPlanFl Oil
  • 60x/1.3 Silicon oil UPlanSApo DIC
  • 60x/1.2 W corr UPlanApo
  • 100x/1.4 oil U PlanSApo DIC

Cameras and control:

  • Andor Ixon3 897 512 EMCCD, 1.2x auxilary magnification
  • OrcaER for widefield imaging on right-side port
  • MetaMorph 7.7 system ID 8562