Spinning disk confocal microscope

Capabilities: Imaging green, red and far-red fluorohores (eg GFP, Alexa 488/568/647) Location: Room C268 LSRC (668-5308) Cost: $15 per hour User manual: pdf | html Request training | Reserve instrument

The spinning disk confocal also has a microinjection system attached to the microscope

Detailed specifications

Olympus IX-70 inverted microscope stand

Perkin Elmer scan head U612B03 (Yokogawa CSU10) with excitation filterwheel

Conventional fluorescence source:

• Lamp housing - Olympus model U-ULH
• Power supply - Olympus model BH2-RFL-T

Conv fluorescence filter:

• Green
• Red

Melles Griot KrAr laser (488, 568, 647 nm) 643-RYB-A01
Omnichrome power supply

Objectives:

• 10x/0.3 (Olympus U PlanFl) px= 0.6584 micron
• 40x/0.6 Ph2 (Olympus LC PlanFl) px= 0.1715 micron
• 40x/0.9 (Olympus UApo) px= 0.1666 micron
• 60x/1.2 W corr (Olympus UPlanApo) px= 0.1081 micron
• 60x/1.4 oil (Olympus Plan Apo) px= 0.1111 micron
• 100x/1.35 oil (Olympus U PlanApo) px = 0.0662 micron

Dichroic(s):

• RGBA polychromatic mirror (spectra available in PE Scan Head Operator's Manual)

Excitation filters:

• Band pass Chroma 80503 (488, 568, 647nm)

Filterwheel controller:

• Sutter Lambda 10-2

Camera/camera controller:

• Hamamatsu Orca-ER monochrome cooled-CCD camera (firewire)

Z-stepper:

• Prior H128E1

Computer:

• Dell Optiplex 745 Intel Core 2 Duo processor E6600 2.40GHz, 4GB RAM, Windows XP Pro 32 bit
• MetaMorph 7.5, system ID 8562

LMCF used to have a second spinning disk which has now been decommissioned and the components put to good use.