Live cell imaging

LMCF has several systems for imaging living mammalian cells at 37C. Samples are best grown in these glass bottom dishes (make sure you get the ones with number 1.5 coverslips). These dishes are good for your cells, fit optimally with the incubators and provide very good focus stability.

Live cell imaging is a balance between getting the information you need and keeping your cells healthy. In general, you will probably have to sacrifice some image quality to minimize photobleaching and phototoxicity. It is important to use as little excitation as possible.

Some people have found this reagent to help with photodamage, its not clear what is actually in it.

Some very useful information is here