Information for publications

To accurately describe the way in which you did your imaging you should include the following information (where relevant)

Below are example starting points for preparing methods sections of papers, which should be expanded with the relevant information outlined above. Further technical details of the microscopes are on their respective pages.

Spinning Disk

Cells were imaged on a spinning disk confocal (Yokogawa CSU10 scanhead) on an olympus IX-70 inverted microscope using 100x/1.35 NA UPlanApo oil objective, 488 nm and 568 nm Kr-Ar laser lines for excitation and ORCA ER CCD camera, the system was controlled by MetaMorph.

Axio Imager

Cells were imaged on a Zeiss Axio Imager upright widefield fluorescence microscope using a 40x/0.75 Plan-NeoFluar objective and green and red filtercubes (chroma). Images were captured on a Hamamatsu ORCA ER CCD camera, the system was controlled by MetaMorph.

SP5 confocal

Cells were imaged on a Leica SP5 confocal microscope using a 40x/1.25 oil objective using 488, 561 and 633 laser lines for excitation. The settings were optimized and the final images scanned with line-averaing of 4.

Live cell station

Cells were imaged at 37C on a Zeiss Axio Observer inverted microscope with stage incubator, CO2 buffering and outer environmental chamber. A Coolsnap ES2 OR QUANTEM EMCCD camera was used for acqusition and the system was controlled by Metamorph.

Acknowledgements

Acknowledgement of LMCF in publications is appreciated and helps to demonstrate our utility to funding sources.