Fluorescence Lifetime Imaging Microscopy (FLIM) System

Fluorescence Lifetime Imaging Microscopy (FLIM) System

Location

RPII 112

Capabilities

  • Time-domain (TCSPC) fluorescence lifetime measurements
  • NDD PMT FLIM detector using a pulsed multiphoton laser on the Olympus FV1000 system
  • SymPhoTime analysis software
  • Intermolecular FRET measurements

Cost

$45.50 per hour base rate, discounted for bulk use

Detailed Specifications

Based on the Olympus FV1000 MPE system

  • Time-correlated single photon counting FLIM measurements
  • Pulsed multiphoton laser provides excitation at 80 MHz (tunable over 680-1080 nm)
  • SymPhoTime software for acquisition and analysis
  • PicoHarp 300 counting electronics