Zeiss 780 Confocal Upright Fixed Stage

Location

RPII 111

Capabilities

  • High sensitivity point scanning confocal imaging
  • Spectral array GaAsP detectors
  • Upright fixed stage microscope
  • In vivo or in vitro samples
  • FCS

Cost

$27 per hour base rate, discounted for bulk use

Priority User Statement

The Zeiss 780 upright confocal was purchased with a shared instrumentation grant for a system for intravital imaging. Users with such experiments receive booking priority on this system in that they may book up to 14 days ahead. All other users with fixed or other live samples are very welcome to use spare time on the system. We will review this policy as the use of the system develops to ensure the system serves its intended use and also more general use in the most efficient manner.

Detailed Specifications

Microscope:

  • Zeiss Axio Examiner with fixed stage (mechanical)
  • 4 Objective turret
  • Metal halide fluorescence source for widefield eyepiece visualization
  • Dapi, Green, Red fluorescence cubes

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 477, 488, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm

Objectives:

  • 2.5x/0.075 Dry EC Plan NeoFluar 440310-9903 WD 9.5 mm (generally in the drawer)
  • 5x/0.15 Dry Plan NeoFluar 440320 WD 18.5 mm (generally in the drawer)
  • 10x/0.45 Dry Zeiss Plan-Apochromat 1063-139 WD 2.0 mm
  • 20x/0.80 Dry Zeiss Plan-Apochromat 440640-9903 WD 0.55 mm
  • 20x/1.0 Water Zeiss W Plan-Apochromat 421452-9800 WD 1.8 mm
  • 63x/1.4 Oil Zeiss Plan-Apochromat 44 07 62 (02) WD 0.19 mm

Dichroic(s):

  • Twin gate dichroic system
  • Low angle of incidence for improved reflection suppression


Spectral array GaAsP detectors

Detectors:

  • 34 channel confocal detector array for versatilely
  • GaAsP high QE 32 channel spectral array with unmixing capability
  • 2 standard alkali PMTs in positions for blue and far-red detection
  • Photon counting possible with GaAsP array and PMT2 (cooled)
  • Transmitted light detector

Computer:

  • Xeon X5260 3.33 Ghz processor, 8 GB RAM, 2TB data drive, 30" monitor
  • Windows 7 SP1 64bit
  • Zen 2010

This system was purchased with funding from the NIH/NCRR Shared Instrumentation Grant 1S10-RR027867-01