Leica SP5 Upright Confocal

Leica SP5 Upright Confocal

Location

Nanline 333D

Capabilities

  • Imaging diverse fluorophores in fixed or live samples
  • Spectrally tunable detectors
  • Ultra-fast resonant scanning (reduced photo-damage with live samples)
  • 3D z-stacks, timelapse, multi-position timelapse

Cost

$30 per hour base rate, discounted for bulk use

Priority User Statement

This system was incorporated into LMCF to make it accessible to all at Duke. To ensure members of the Kuo, Bagnat and Eroglu labs have access to the machine they choose to share, they are able to book time up to 14 days ahead; other users can make reservations up to 7 days ahead.

Detailed Specifications

Microscope:

  • Leica DM6000CS
  • Metal halide fluorescence source
  • Motorized scanning stage

Tandem scanners (select at software initialization)

  • Conventional scanner
  • Resonant scanner (8 000 Hz)

Lasers:

  • 405 nm Diode laser
  • Argon (458, 488, 476nm, 514nm)
  • 561 nm Diode laser
  • HeNe 633nm

Conventional fluorescence filters:

  • Analyzer
  • Dapi
  • Green
  • Red

Objectives:

  • 10x/0.40 10X HC PL APO CS, NA:0.40, air, WD: 2.2mm, coverglass: 0.17mm, M25
  • 20x/0.70 HC PL APO CS IMM, NA: 0.7, DIC, WD: 0.59mm, coverglass: 0.17mm, Thread type: M25.
  • 40x/1.25-0.75 oil : 40X, HCX PL APO, NA: 1.25, Oil, DIC, WD: 0.1mm, coverglass: 0.17mm, Iris diaphragm, Thread type: M25
  • 63x/1.4-0.60 oil : HCX PL APO, NA: 1.4, Oil, DIC, WD: 0.09mm, Coverglass: 0.17mm, Iris Diaphragm, Thread type: M25

Dichroic(s):

  • RT 30/70
  • Substrate
  • TD 488/561/633
  • DD 458/514
  • RSP 500
  • DD 488/561

Detectors:

  • Four channels
  • All cooled PMTs
  • All spectral detactors
  • Transmitted light detector

Computer:

  • HP xw8400 workstation with Intel Xeon 5160 CPU, 4 GB RAM, OS and data drives
  • Windows xp
  • Leica LAS AF 2.3