Past news and events . . .

New Olympus Andor XD revolution spinning disk is installed

This new system in installed in C142 LSRC and is ready for use - please request training here. Spinning disk confocal provides fast optical sectioning with low levels of photodamage. The system has 405, 445, 488, 515 and 561 nm laser lines, an EMCCD camera, a range of objectives, stage incubation and hardware autofocus. The system is controlled by metamorph and should be an easy learning experience for users who have used our live cell stations before.

Laser capture and microdissection

A laser microdissection and capture system will be available from early 2012. This resource allows the physical isolation of small regions of tissues for analysis. A UV laser physically cuts around the region you define and the fragment can be catapulted into a tube for analysis by a variety of molecular techniques.

Many thanks to the DHVI for the funding for this equipment and generously sharing this resource with all at Duke. This is now installed and available.

CMBI Conference: Frontiers in Optical Imaging: Data Collection and Data Mining, from the Clinic to the Art Gallery

The 2012 CMBI conference will be held in FFSC 9 & 10 December. Registration is free.

SP2 confocal to be upgraded

The Leica SP2 confocal will be upgraded/replaced towards the end of 2012. We will be testing some upright confocal systems in the coming months, including . . .

Leica SP8 demo

Leica will bring their new SP8 confocal to Duke 28-30 August 2012. This system has several improvements over the SP5 confocal, including hybrid detectors that offer improved sensitivity over the standard PMTs in confocals.

Please let Sam know if you would like to test this system with your samples.

Microscopy and Image Analysis Classes: two short courses in August

Principles of microscopy - a two afternoon overview held on 7th and 9th of August

Image analysis - An interactive one morning class on 15th August covering image processing, visualization and analysis

Email Sam if you would like to attend either the next time the classes are run.

Lasers and Microscopy user group meeting at UNC

A free informal meeting centered around microscopy was held at UNC august 9 2012.

Booking calendar change

The LMCF booking calendar was migrated to a new server on 7/24/12. The URL has changed so you may want to update your bookmarks although the old page redirects to the new one.

Image Analysis Class

The first LMCF short course in image analysis was held 17 May 2012. The class will be run again in the summer so please email Sam if you would like to attend.

Leica GSD super resolution demo

Leica brought a super resolution system to Duke April 2 to 11 2012. The system is based on a widefield or TIRF based acquisition. Please email Sam if you are interested in trying the system. It will be in 472 Sands.

Benjamin Carlson joins LMCF

Please welcome Ben as he starts work in LMCF on 9 January 2012. Ben will be based in the Nanaline Duke suite now part of LMCF. He will provide local support for those resources and will also be working on other systems across LMCF. Ben did his PhD at Duke in the Cell Biology department and brings a lot of imaging experience and many different skills to LMCF. Having a third person working in the facility will allow us to thoroughly support our growing number of systems and develop new capabilities.

New Spinning Disk system ordered

LMCF inherited a basic spinning disk system which lacked the features to make it useful for advanced live cell imaging. A new system has been ordered and should arrive early 2012 and will go in C142 (TIRF will be moved to RP2). The Andor Revolution XD system consists of a CsuX1 scanhead on an Olympus IX81 stand with relfection based autofocus, Andor iXon 3 897 EMCCD, 405, 442, 488, 515 and 561 nm laser lines, stage incubation and a range of objectives including a silicon oil lens matched to the RI of many tissues.

Cell Biology's imaging facility integrated with LMCF

Cell Biology has maintained an imaging facility for departmental use for a number of years. This will be joined to and managed as part of LMCF effective 1 November 2011 to provide a wide array of imaging resources to the whole campus through a single core resource. In the coming months we plan to hire a third person in LMCF to be based principally in Nanaline Duke to provide full support for the systems.

These systems in Nanaline Duke are now available to all (Members of the Cell Biology Department have some booking priority on these systems in recognition of the Cell Biology department's contribution in purchasing them).

As always, if you would like to use any of these system please fill out the training request form.

If you are current user of the above scopes and need access to the new booking system please fill out the same form and indicate which system(s) you are already trained on

AMG EVOS on loan to LMCF

This system is available in LMCF. We hope you can try it and let us know what you think. It is very easy to use so ideal for checking transfection of cells and assessing your slides.

VivaView Incubator microscope purchased with a NCBC grant

Following award of an NCBC Institutional Development Grant, LMCF has acquired an Olympus VivaView incubator microscope. The system is essentially a microscope built inside a TC incubator offering optimal conditions for long-term imaging of livings cells. The system can take DIC and fluorescence images of up to 8 dishes of cells and is ideal for relatively slow, long-term experiments. (Standard live cell systems offer more flexibility and work well for shorter periods e.g. overnight experiments).

The system is setup in RP2 where we have a TC hood to help with sample preparation. Some pictures are on this page.

The system uses MetaMorph for acquisition and a new workstation is now available in RP2 to help with data analysis and movie making. This workstation (very originally called "RP2 workstation") also has a copy of Imaris for visualization and quanitification of 3D data.

New Equipment for LMCF

Another high-end image analysis workstation with Imaris software is now installed in LMCF and the existing workstations have been reorganized slightly. The new Imaris software offers very powerful analysis and visualization resources and has the modules Imaris, MeasurementPro, Imaris Track, Imaris Colocalization, In Press, Filament Tracer and Imaris XT. Thanks to the Duke Cancer Center for funding for this new resource.

NIH shared instrumentation grants and internal funding will enable the installation of the following equipment

  • DeltaVision - a widefield imaging system similar to the live cell station but with a few extra capabilities. It offers very fast multichannel imaging, optimized deconvolution, high-precision stage, hardware autofocus and directable lasers for photoactivation and photobleaching experiments. Installed
  • Zeiss 780 confocal featuring high sensitivity GaAsP spectral detectors. It is an upright system optimized for imaging in vivo samples, but when not being used for such experiments the system will be available for other applications which may benefit from the features of this system. Installed
  • Olympus FV1000 multiphoton - Optimized multiphoton imaging with 4 non-descanned detectors. The system will enable imaging of thick fixed and living samples to a greater depth than is possible using a standard confocal. The system will also have FLIM capability useful for measuring intermolecular FRET. Installed
  • TCSPC Fluorescence Lifetime Imaging Microscopy (FLIM) capability - PicoQuant SymPhoTime system. Installed

New Space for LMCF

LMCF have been given two large rooms in Research Park II. This new site will be a very convenient location for the equipment optimized for in vivo imaging as it is very close to animal facilities and re-entry to housing will be possible allowing longitudinal studies. The location will also be open to fixed and non-animal live samples.

This additional space in RPII will allow LMCF to acquire more equipment for additional capabilities and capacity in the future. As always, we welcome your ideas and suggestions for what new resources would be useful.

Spinning Disk demo

Perkin Elmer will bring the latest version of their UltraView spinning disk system to Duke for demonstration available 1-3 February 2011. LMCF has a basic spinning disk system which needs upgrades and improvement to be useful. It will be useful to see if a state-of-the-art spinning disk system would be valuable system to have in the facility and if it is something we should try and acquire. I hope you can come and test the system with your samples, please email Sam to arrange a time. The system will be setup in 111 RP2.

The system will be a full UltraVIEW VoX with . . .

  • 6 laser lines (405/440/488/515/561/640)
  • Motorized XY Stage
  • Stage Mounted Piezo Z
  • Proprietary 1Kx1K EMCCD camera
  • Nikon fully motorized inverted microscope
  • Stage top incubator
  • Volocity acquisition software

 

Cytoo Seminar: The Use of Micropatterns for Quantitative Cell Analysis

Pauline Menager PhD from Cytoo will present an overview of the experimental possibilities of adhesive micropatterns. 12 noon Biological Science 144 Thursday 16th December 2010. Pizza will be served (please RSVP to Brian Carmichael).

Learn more about Cytoo's technology at http://www.cytoo.com | Event flyer

cytoo

From Imaging to Understanding: Visualization and Smart Analysis

The 2010 annual meeting of Duke's Center for Molecular and Biomolecular Imaging will focus on the interdisciplinary intellectual challenges associated with extracting useful information from raw data in imaging and spectroscopy.

December 12-13, 2010, French Family Science Center | Event flyer

Molecular Probes seminar: Emerging Technologies in Cellular Analysis & Imaging

Magnus Persmark PhD from Molecular Probes will present a seminar at 1000 on Monday 6th December 2010 in A247 LSRC describing some of the existing and new tools and reagents available for fluorescence imaging -"From Foundational Tools to Next Generation Tools for Live Cell and Fluorescence Imaging". Topics to be covered include

  • Fluorescence Tools for Optimal Sub-cellular Analysis
  • Novel Tools for Cellular Analysis: Cell Health and High Content Assays
  • Assays for Cellular Ion Flux: Combining Sensors with BacMam Transient Gene Delivery
  • Visualization of Endocytosis and Phagocytosis

Event flyer

Please RSVP to dean.gillespie@lifetech.com if you would like to attend so they can ensure adequate quantities of refreshments are provided.

Leica STED demo in August 2010

Leica will bring their new CW-STED system to Duke August 2-12 2010. STED improves the XY resolution of confocal imaging about three times. Further details about the event, technique and sample prep are here.

New Imaris workstation and Imaris user group meeting at Duke in May

The Imaris user group meeting will be hosted by LMCF at Duke University May 11-13 2010. This is a hands-on 3 day course for learning more about this powerful analysis software that will soon be available in LMCF. More details and registration on the Imaris website. (The event is free of charge for Duke people).

Two promising scores for Shared Instrumentation Grants

LMCF submitted two Shared Instrumentation Grants in March 09 which are in strong positions for funding. These grants are for:

  • Intravital confocal/multiphoton - an upright fixed stage system optimized for in vivo imaging with both conventional confocal and multi-photon capabilities.
  • Live cell system with capabilities for photoactivation/conversion and FRAP experiments.

We hope to finalize these outcomes and decisions soon.

Olympus Demo

We will be looking again at two olympus systems for live cell imaging.

VivaView LCV – A microscope inside an incubator for long term imaging. Here March 25 to April 9 2010.

IX81 with environmental chamber etc (similar to the LMCF live cell station) - Here March 29 to April 9 2010.

School of Medicine Core Facility Voucher Program

Faculty in the Medical School can apply for up to $10,000 to fund a project's use of LMCF or the other core facilities at Duke. Investigators must prepare a 1-page proposal describing the research question to be investigated, rationale, proposed plan, and justification for amount requested. The deadline for submission is March 31, 2010.

Seminar: "Super Resolution and High Speed Imaging with Delta Vision OMX"

Paul Goodwin (Technical Fellow, Applied Precision) will present details of the Applied Precision OMX super resolution system. This uses 3D structured illumination to double the resolution of conventional microscopy, with improvement in lateral and axial directions.

A247 LSRC, 11:00 am Friday 29 January 2010

Short course in microscopy in January 2010

If you would like to go over a broad overview of microscopy as two afternoon-long classes the microscopy short-course will be held on January 7 and 8 at 2pm each day. It is held for a small group with plenty of chances to ask questions as we go.

CMBI meetings December 2009

Duke's Center for Molecular and Biomolecular Imaging is partnering with Coherent, Inc. for two meetings at Duke:

New Directions in Microscopy: Sharper, Deeper, Smarter
December 14-15, 2009
Southeast Regional Ultrafast Meeting
December 15-16, 2009

Optical microscopy has been an essential tool in biology and medical practice for centuries. As imaging technologies evolve from monitors of structure to monitors of function, optical microscopies have also evolved dramatically, driven largely by innovations in laser technology. This symposium will focus on new optical imaging methods which can

  • Improve resolution by an order of magnitude or more past the traditional diffraction limit
  • Overcome scattering and absorption in tissue to produce high resolution images far deeper in tissue than previously possible
  • Create entirely new contrast in images, which correlates better with the intrinsic cellular biochemistry (for example, of cancer)

Invited presentations will address multiple new methods in superresolution microscopy, nonlinear imaging (two-photon microscopy, with and without fluorescence signatures), and effects of spatial and polarization shaping on the laser to reduce scattering and improve penetration depth. Both in vivo and in vitro applications will be presented. The meeting will include laboratory tours of the University shared microscopy facility in French, and various laser laboratories. The Plenary speakers will be Michael Fayer (Stanford) and Tuan vo-Dinh (Duke); invited speakers include Jerome Mertz (Boston University), David Smith (Duke), Joseph Izatt (Duke), Michael Levene (Yale), Martin Fischer (Duke), Lin Shao (Janelia Farm, HHMI), Warren Zipfel (Cornell), Kelly Nelson (Duke), Sam Johnson (Duke), and Volker Westphal (Gottingen).

This year, our meeting will dovetail with the Southeast Regional Ultrafast Laser meeting, sponsored by Coherent. CMBI speakers will present on Monday and Tuesday; the Ultrafast meeting will begin Tuesday afternoon and continue on Wednesday, with dinner and an afterdinner presentation, "Breasts and Brains, Similarities and Differences: How Lasers fit into Molecular Imaging" from Warren S. Warren (Duke) on Tuesday night.

Olympus Confocal Imaging Demonstration

  • FV10i Confocal – Self-contained confocal with 4 LD lasers, 2 spectral-based PMTs, oil objectives, motorized stage, and self-contained anti-vibration system for use by individuals or as a workhorse system for standard confocal imaging.
  • Fluoview FV1000 Confocal – Spectral confocal with SIM scanner.
  • Olympus DSU (Disk Scanning Unit) – The Olympus DSU comes with 5 disks with various slit openings for use with a variety of objectives from 10x to 100X. Well suited for developmental biology samples.

Tuesday December 15th, 2009

  • 8am – 12pm – FV1000 Confocal and Olympus DSU Open House
  • 1pm – 5pm – FV10i Open House
  • 1pm – 5pm – Personalized demonstrations on the Olympus DSU

Wednesday December 16th through Friday December 18th

Personalized demonstrations of all equipment.

Olympus Live Cell & Widefield Imaging Demonstration

  • VivaView LCV – Live cell viewer (Time-Lapse Imaging DIC/ Fluorescence Incubator Microscope): Perform Long-Term Multidimensional Time-Lapse Imaging on up to Eight Dishes with Multi-Points, Multi-Color and XYZ on each dish.
  • FSX100 – Self-contained, computer-driven microscope for obtaining high-resolution images of brightfield and fluorescence slides.
  • MVX-10 Research Macro Zoom Fluorescence Microscope. Fluorescence imaging at Macro as well as Micro with high N.A. objectives

Tuesday October 27th, 2009

  • 8am – 12pm – VivaView LCV open house
  • 1pm – 5pm – Full open house
  • 4pm – 5pm – Overnight setup of LCV for specific customers (up to eight 35mm coverslip bottom plates (Lab-Tek preferred brand)

Wednesday October 27th (8am) through Friday October 29th (noon)

Personalized demonstrations of all equipment, including overnight timelapse imaging of up to eight 35mm cover slip bottom dishes.

 

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