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Leica GSD super resolution demo
Leica will bring a super resolution system to Duke April 2 to 11 2012. The system is based on a widefield or TIRF based acquisition and details of the system and the principles of its operation can be found here.
Specific sample preparation is required. Protocols and sample preps: pdf | link to Leica
Please email Sam if you are interested in trying the system. It will be in 472 Sands.
New Olympus Andor XD revolution spinning disk is installed
This new system in installed in C142 LSRC and is ready for use - please request training here. Spinning disk confocal provides fast optical sectioning with low levels of photodamage. The system has 405, 445, 488, 515 and 561 nm laser lines, an EMCCD camera, a range of objectives, stage incubation and hardware autofocus. The system is controlled by metamorph and should be an easy learning experience for users who have used our live cell stations before.
Benjamin Carlson joins LMCF
Please welcome Ben as he starts work in LMCF on 9 January 2012. Ben will be based in the Nanaline Duke suite now part of LMCF. He will provide local support for those resources and will also be working on other systems across LMCF. Ben did his PhD at Duke in the Cell Biology department and brings a lot of imaging experience and many different skills to LMCF. Having a third person working in the facility will allow us to thoroughly support our growing number of systems and develop new capabilities.
Laser capture and microdissection
A laser microdissection and capture system will be available from early 2012. This resource allows the physical isolation of small regions of tissues for analysis. A UV laser physically cuts around the region you define and the fragment can be catapulted into a tube for analysis by a variety of molecular techniques.
Many thanks to the DHVI for the funding for this equipment and generously sharing this resource with all at Duke. This is now installed and available.
New Spinning Disk system ordered
LMCF inherited a basic spinning disk system which lacked the features to make it useful for advanced live cell imaging. A new system has been ordered and should arrive early 2012 and will go in C142 (TIRF will be moved to RP2). The Andor Revolution XD system consists of a CsuX1 scanhead on an Olympus IX81 stand with relfection based autofocus, Andor iXon 3 897 EMCCD, 405, 442, 488, 515 and 561 nm laser lines, stage incubation and a range of objectives including a silicon oil lens matched to the RI of many tissues.
Cell Biology's imaging facility integrated with LMCF
Cell Biology has maintained an imaging facility for departmental use for a number of years. This will be joined to and managed as part of LMCF effective 1 November 2011 to provide a wide array of imaging resources to the whole campus through a single core resource. In the coming months we plan to hire a third person in LMCF to be based principally in Nanaline Duke to provide full support for the systems.
These systems in Nanaline Duke are now available to all (Members of the Cell Biology Department have some booking priority on these systems in recognition of the Cell Biology department's contribution in purchasing them).
As always, if you would like to use any of these system please fill out the training request form.
If you are current user of the above scopes and need access to the new booking system please fill out the same form and indicate which system(s) you are already trained on
AMG EVOS on loan to LMCF
This system is available in LMCF. We hope you can try it and let us know what you think. It is very easy to use so ideal for checking transfection of cells and assessing your slides.
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