SPIM/Light Sheet and super-resolution systems at Duke

We have the opportunity to test systems offering new ways of imaging. In May Zeiss will bring a light sheet system to campus. Over the summer we will have two different 3D super-resolution systems to try with your samples. Please see this page for more details.

A Leica SP8 confocal is now available in LMCF

An upright Leica SP8 confocal is now available in 337 Nan Duke. It features sensitive hybrid detectors, a resonant scanner and several other features and capabilities. .

A second Zeiss 780 now available in LMCF

An inverted Zeiss 780 confocal is now available to users as part of LMCF. The system was purchased by Vann Bennett who has generously shared the system through LMCF.

ScaleView capability added to MultiPhoton

The Olympus multiphoton has been upgraded with a motorized stage and stitching software and an objective lens optimized for imaging samples optically cleared with the ScaleView reagent offering imaging to a depth of 8 mm. Have a look at what can be achieved with this technique and system here.

Rockland antibodies

Free samples of Rockland primary antibodies - you get to try the antibody; they want example images.

What you can do in LMCF

Download this poster to see an overview of the capabilities and equipment available in LMCF.

New Olympus Andor XD revolution spinning disk is installed

This new system in installed in C142 LSRC and is ready for use - please request training here. Spinning disk confocal provides fast optical sectioning with low levels of photodamage. The system has 405, 445, 488, 515 and 561 nm laser lines, an EMCCD camera, a range of objectives, stage incubation and hardware autofocus. The system is controlled by metamorph and should be an easy learning experience for users who have used our live cell stations before.

Laser capture and microdissection

A laser microdissection and capture system will be available from early 2012. This resource allows the physical isolation of small regions of tissues for analysis. A UV laser physically cuts around the region you define and the fragment can be catapulted into a tube for analysis by a variety of molecular techniques.

Many thanks to the DHVI for the funding for this equipment and generously sharing this resource with all at Duke. This is now installed and available.