Live cell station 2: Zeiss Axiovert 200 M

Capabilities: Imaging living samples - any fluorescent protein Temperature, humidity, CO2 control Multiposition timelapse Location: Room 337B Nanaline Duke (681-4076) Cost: $13.40/hr for first 3 hours, then $2/hr base rate, discounted for bulk use User manual: Request training | Reserve instrument

Detailed specifications

Zeiss AxioVert 200 Motorized

• Opto-electronically encoded motorized XY stage 120*100 mm travel.
• X-CITE 120 PC metal halide fluorescence light source
• Uniblitz shutter
• Camera: Orca ER

Filter cubes:

• DIC
• GFP (Endow)
• Texas Red (XF108-2)
• YFP (Omega XF104)
• CFP

Objectives:

• 2.5x/0.075 Plan Neofluar 44 01 35 9.3 mm WD (pixel size = 2.386 microns)
• 10x/0.5 Fluar 44 03 10 2 mm WD (pixel size = 0.530 microns)
• 5x/0.15 EC Plan Neofluar Ph1 440321-9901 13.6 mm WD (pixel size = 1.248 microns)
• 63x/1.4 oil Plan-Apochromat DIC 1113-108 (pixel size = 0.00996 microns)
• 20x/0.4 LD Plan-Neofluar Korr 0-1.5 441340-9970 (pixel size = 0.309 microns)
• 40x/0.85 Plan Neofluar Pol 44 03 53 (pixel size = 0.156 microns)

Incubation:

• Pecon XL S1 incubator and control modules
• Heating Insert P - 35 mm glass bottom dishes
• Heating insert M06 - 6 well plates
• Heating insert M12 - 12 well plates

Computer:

• Dell Precision T3400 4GB RAM, Dual hard-drives
• Windows 7 32 bit
• MetaMorph 7.7.5, system ID 9252