Live cell station 2: Zeiss Axiovert 200 M

Duke lmcf live cell station 2 Capabilities:
  • Imaging living samples - any fluorescent protein
  • Temperature, humidity, CO2 control
  • Multiposition timelapse

Location: Room 111 of Research Park 2

Cost: $14/hr for first 3 hours, then $2/hr base rate, discounted for bulk use

User manual:

Request training | Reserve instrument

Detailed specifications

Zeiss AxioVert 200 Motorized

  • Opto-electronically encoded motorized XY stage 120*100 mm travel.
  • X-CITE 120 PC metal halide fluorescence light source
  • Uniblitz shutter
  • Camera: Orca ER

Filter cubes:

  • DIC
  • GFP (Endow)
  • Texas Red (XF108-2)
  • YFP (Omega XF104)
  • CFP

Objectives:

  • 2.5x/0.075 Plan Neofluar 44 01 35 9.3 mm WD (pixel size = 2.386 microns)
  • 10x/0.5 Fluar 44 03 10 2 mm WD (pixel size = 0.530 microns)
  • 5x/0.15 EC Plan Neofluar Ph1 440321-9901 13.6 mm WD (pixel size = 1.248 microns)
  • 63x/1.4 oil Plan-Apochromat DIC 1113-108 (pixel size = 0.00996 microns)
  • 20x/0.4 LD Plan-Neofluar Korr 0-1.5 441340-9970 (pixel size = 0.309 microns)
  • 40x/0.85 Plan Neofluar Pol 44 03 53 (pixel size = 0.156 microns)

Incubation:

  • Pecon XL S1 incubator and control modules
  • Heating Insert P - 35 mm glass bottom dishes
  • Heating insert M06 - 6 well plates
  • Heating insert M12 - 12 well plates

Computer:

  • Dell Precision T3400 4GB RAM, Dual hard-drives
  • Windows 7 32 bit
  • MetaMorph 7.7.5, system ID 9252