Live cell station: Zeiss Axio Observer

Duke LMCF live cell station zeiss axio observer Capabilities:
  • Imaging living samples - any fluorescent protein
  • Temperature, humidity, CO2 control
  • High sensitivity - EMCCD camera
  • Multiposition timelapse
  • Tiled images

Location: C142 LSRC (613-8217)

Cost: $14/hr for first 3 hours, then $2/hr base rate, discounted for bulk use

User manual: Using MetaMorph for time-lapse imaging | Using ZEN for tile imaging

Request training | Reserve instrument

Detailed specifications

Zeiss Axio Observer Z1 motorized

Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and plates.

Pecon XL S1 incubator and control modules

X-CITE 120XL metal halide fluorescence light source

Filter cubes:

  1. Analyzer
  2. DAPI filtercube 49 (BP G365, FT 395, BP 445/50)
  3. CFP filtercube HE47(BP 436/25, FT 455, BP 480/40)
  4. GFP filtercube HE38 (BP 470/40, FT 495, BP 525/50)
  5. YFP filtercube HE46 (BP 500/20, FT 515, BP 535/30)
  6. Red filtercube HQ45 (BP 560/40, FT 585, BP 630/75)

FRET cube available (Semrock x438/24, FF458-Di01, m542/27) - swap into position 4 for ratiometric CFP/YFP FRET

Cy5 cube available (Semrock FF01-630/38, FF655-Di01, FF01-694/44)

Fluorescence attenuator:

  • Open (100% Transmission)
  • 70% T
  • 50% T
  • 40% T
  • 20% T
  • 2% T

Objectives:

  • 5x/0.16 Plan-NeoFluar (440320-9902) WD: 18.5mm
  • 10x/0.30 Plan-NeoFluar Ph1 (440331-9902) WD: 5.2mm
  • 20x/0.80 Plan-Apo DIC (440640-9903) WD: 0.55mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
  • 40x/0.75 EC-Plan-NeoFluar DIC (440350-9903), WD 0.71mm Thread type: M0.8X1/36”
  • 63x/1.4 oil Plan Apochromat DIC (440762-9904) WD: 0.19mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
  • 100x/1.46 oil Plan Apochromat DIC (420792-9800) WD: 0.11mm, Coverglass: 0.17mm, (UV)VIS-IR, Thread type: M27X0.75
  • Available: 40x/1.3 oil Plan-NeoFluar DIC (440452-9903) WD: 0.20mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”

Calibrations for Photometrics Evolve EMCCD and CoolSNAP ES2 cameras:

  • 5x EM px= 3.0828 um, CoolSNAP px= 1.2645 um
  • 10x EM px= 1.5556 um, CoolSNAP px= 0.6135 um
  • 20x EM px= 0.7726 um, CoolSNAP px= 0.3091 um
  • 40x EM px= 0.3810 um, CoolSNAP px= 0.1570 um
  • 63x EM px= 0.2423 um, CoolSNAP px= 0.0983 um
  • 100x EM px= 0.1558 um, CoolSNAP px=0.0629 um

Cameras:

  • Photometrics Evolve back-thinned EM-CCD camera (512*512)
  • Photometrics CoolSNAP ES2 CCD camera (1392*1040)

Computer:

  • Dell Precision T7000, 16 GB RAM
  • Windows 7 64 bit
  • MetaMorph 7.8, system ID 32330
  • ZEN Pro 2.3