Live cell station: Zeiss Axio Observer
 |
Capabilities:
Location: C142 LSRC (613-8217) Cost: $13.40/hr for first 3
hours, then $2/hr base rate, discounted for bulk use |
Detailed specifications
Zeiss Axio Observer Z1 motorized
Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and plates.
Pecon XL S1 incubator and control modules
X-CITE 120XL metal halide fluorescence light source
Filter cubes:
- Analyzer
- DAPI filtercube 49 (BP G365, FT 395, BP 445/50)
- CFP filtercube HE47(BP 436/25, FT 455, BP 480/40)
- GFP filtercube HE38 (BP 470/40, FT 495, BP 525/50)
- YFP filtercube HE46 (BP 500/20, FT 515, BP 535/30)
- Red filtercube HQ45 (BP 560/40, FT 585, BP 630/75)
FRET cube available (Semrock x438/24, FF458-Di01, m542/27) - swap into position 4 for ratiometric CFP/YFP FRET
Fluorescence attenuator:
- Open (100% Transmission)
- 70% T
- 50% T
- 40% T
- 20% T
- 2% T
Objectives:
- 5x/0.16 Plan-NeoFluar (440320-9902)
- 10x/0.30 Plan-NeoFluar Ph1 (440331-9902)
- 20x/0.80 Plan-Apo DIC (440640-9903) WD: 0.55mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
- 40x/1.3 oil Plan-NeoFluar DIC (440452-9903) WD: 0.20mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
- 63x/1.4 oil Plan Apochromat DIC (440762-9904) WD: 0.19mm, Coverglass: 0.17mm, Thread type: M0.8X1/36”
- 100x/1.46 oil Plan Apochromat DIC (420792-9800) WD: 0.11mm, Coverglass: 0.17mm, (UV)VIS-IR, Thread type: M27X0.75
Calibrations for QuantEM (EM) and CoolSnap (ES) cameras:
-
5x EM px= 3.0828 um, ES px= 1.2645 um
-
10x EM px= 1.5556 um, ES px= 0.6135 um
-
20x EM px= 0.7726 um, ES px= 0.3091 um
-
40x EM px= 0.3810 um, ES px= 0.1570 um
-
63x EM px= 0.2423 um, ES px= 0.0983 um
-
100x EM px= 0.1558 um, ES px=0.0629 um
Cameras:
- Coolsnap ES2 high resolution CCD camera
- QuantEM backthinned EM-CCD camera (512*512)
Computer:
- Dell Precision 690 Dual Core Intel Xeon processor 5140, 4GB RAM, Dual hard-drives
- WindowsXP Professional SP2 32 bit
- MetaMorph 7.6.5, system ID 32330