Live cell station: Zeiss Axio Observer

Duke LMCF live cell station zeiss axio observer Capabilities:
  • Imaging living samples - any fluorescent protein
  • Temperature, humidity, CO2 control
  • High sensitivity - EMCCD camera
  • Multiposition timelapse
  • Tiled images

Location: C142 LSRC

Cost: $12/hr for first 2 hours, then $2/hr

User manual: pdf | html

Request training | Reserve instrument

Detailed specifications

Zeiss Axio Observer Z1 motorized

Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and plates.

Pecon XL S1 incubator and control modules

X-CITE 120XL metal halide fluorescence light source

Filter cubes:

  1. Analyzer
  2. DAPI filtercube 49
  3. CFP filtercube HE47
  4. GFP filtercube HE38
  5. YFP filtercube HE46
  6. Red filtercube HQ45

Fluorescence attenuator:

  • Open (100% Transmission)
  • 80% T
  • 60% T
  • 40% T
  • 20% T
  • 2% T

Objectives:

  • 5x/0.16 Plan-NeoFluar (440320-9902)
  • 10x/0.30 Plan-NeoFluar Ph1 (440331-9902)
  • 20x/0.80 Plan-Apo DIC (440640-9903)
  • 40x/1.3 oil Plan-NeoFluar DIC (440452-9903)
  • 63x/1.4 oil Plan Apochromat DIC (440762-9904)
  • 100x/1.46 oil Plan Apochromat DIC (420792-9800)

Calibrations for QuantEM (EM) and CoolSnap (ES) cameras:

  • 5x EM px= 3.0828 um, ES px= 1.2645 um
  • 10x EM px= 1.5556 um, ES px= 0.6135 um
  • 20x EM px= 0.7726 um, ES px= 0.3091 um
  • 40x EM px= 0.3810 um, ES px= 0.1570 um
  • 63x EM px= 0.2423 um, ES px= 0.0983 um
  • 100x EM px= 0.1558 um, ES px=0.0629 um

Cameras:

  • Coolsnap ES high resolution CCD camera
  • QuantEM backthinned EM-CCD camera (512*512)

Computer:

  • Dell Precision 690 Dual Core Intel Xeon processor 5140, 4GB RAM, Dual hard-drives
  • WindowsXP Professional SP2 32 bit
  • MetaMorph 7.5, system ID 32330