Fluorescence Lifetime Imaging Microscopy (FLIM) system

Duke LMCF FLIM Capabilities:
  • Time-domain (TCSPC) fluorescence lifetime measurements
  • NDD PMT FLIM detector using a pulsed multiphoton laser on the Olympus FV1000 system
  • SymPhoTime analysis software
  • Intermolecular FRET measurements

Location: 112 Research Park II (613-8219)

Cost: $41.20/hr base rate, discounted for bulk use

User manual:

Request training | Reserve instrument

Detailed specifications

Based on the Olympus FV1000 MPE system

  • Time-correlated single photon counting FLIM measurements
  • Pulsed multiphoton laser provides excitation at 80 MHz (tunable over 680-1080 nm)
  • SymPhoTime software for acquisition and analysis
  • PicoHarp 300 counting electronics