Preparing your samples

We have some general advice about this here.

If you are making slides, using the correct coverslip is very important. Number 1.5 coverslips are optimal for most objectives. Using the wrong ones can make the images very dim and imperfect, and may even make the slides unimagable.

If you are using live cells, glass bottom dishes work well. Again the ones with number 1.5 coverslips are generally optimal and the catalog number is P35G-1.5-14-C.

There are many secondary antibodies to choose from, Alexa488 and Alexa568 are very good green and red ones and they will work with most microscopes in the facility.