A Basic introduction to microscopy:
Spinning disk confocals
Two photon excitation
Preparing your samples
Fluorescence microscopes produce widefield images. This is the basis of what happens in a fluorescence microscope:
This is what an upright fluorescence scope typically looks like. Notice the fluorescence light path is only above the stage (this is called epi-illumination and the objective is both the condenser and the imaging lens). The transmitted light path features a condenser on one side of the sample and the objective on the other.