A Basic introduction to microscopy: Introduction Microscope configurations Transmitted light Fluorescence Fluorescence microscopes Stereoscopes Confocals Spinning disk confocals Two photon excitation TIRF Preparing your samples Objectives Images Deconvolution Image analysis Other points/FAQs 
Deconvolution This is a mathematical processing of images to reduce the blur from out of focus light. This increases the signal:noise ratio of the image. There are two main types of deconvolution: Deblurring/nearest neighbor uses a mathematical process to estimate the blur from other focal planes and remove it. This sharpens the image but is nonquantitative. Restoration is more computational intensive and works best with 3D data sets. The software uses an iterative mathematical process to reassign the photons.
