A Basic introduction to microscopy:
Spinning disk confocals
Two photon excitation
Preparing your samples
This is a mathematical processing of images to reduce the blur from out of focus light. This increases the signal:noise ratio of the image. There are two main types of deconvolution:
Deblurring/nearest neighbor uses a mathematical process to estimate the blur from other focal planes and remove it. This sharpens the image but is non-quantitative.
Restoration is more computational intensive and works best with 3D data sets. The software uses an iterative mathematical process to reassign the photons.