A Basic introduction to microscopy:
Spinning disk confocals
Two photon excitation
Preparing your samples
You've probably done this type of microscopy looking at tissue culture cells. In brightfield, light passes through the specimen, some is absorbed/scattered and an image is formed from what passes through.
Contrast is improved with techniques such as phase contrast or DIC. Tissue samples can be stained with dyes (eg H&E) or with immuno-histochemistry and colour images can be captured on either the Axio Imager or the stereoscope.