Laser lines and filters available

The facility has a large variety of laser lines, excitiation and emission filters available across a wide range of microscopes. This table is to help you quickly find the microscopes able to image a specific fluorophore. For further details see the specification page for each scope (links on the menu on the left).

Laser line (nm) Confocal system(s) on which it is available
405 510 inverted, SP5s, SP8, 710, 780s and TIRF. Deltavision point activation laser
445 SD
458 510s, SP5s, SP8, 710, 780s
477 510s, SP5s, 710, 780s
488 All confocals, SD and TIRF. Deltavision point activation/bleaching laser
496 Leica SP5s
514 510s, SP5s, SP8, 710, 780s, SD
543 510 upright
561 510 inverted, SP5s, SP8, 710, 780s, SD and TIRF
594 510 inverted, 710 and SP5 inv
633 All scanning confocals
635 TIRF
680-1080 Multiphoton

Numerous emission filters are available for the confocals and are described on the individual equipment pages. Spectral detection is available on the SP5/SP8 (serial) and 710/780 (parralel).

  Widefield system
DAPI Live cell, DeltaVision and Axio Imager/Observer
CFP Live cell, DeltaVisio and Axio Imager/Observer
GFP Live cell, DeltaVisio and Axio Imager/Observer
YFP Live cell, DeltaVisio and Axio Imager/Observer
Red Live cell, DeltaVisio and Axio Imager/Observer
Far-red Axio Imager (filterwheel) / Observer (available cube)
Cy7 Have cube (generally with Axio Observer)

Exact wavelengths of the filters are available on the individual equipment pages.