Zeiss laser microdissection and capture

Duke LMCF laser capture Capabilities:
  • Cut and extract regions of a tissue
  • UV (355 nm) cutting beam
  • Non-contact capture
  • Also provides stitched imaging of large areas with fluorescence and color camera imaging

Location: Room 111 Research Park II (613-8219)

Cost: $20/hr discounted for bulk use

User manual: html

Request training | Reserve instrument

This system was purchased with NIH funding awarded to CHAVI and DHVI , who kindly donated the system to LMCF so all at Duke would be able to use this system and technology.

Information on how the system works

Detailed specifications

PALM MicroBeam 4.2

Robomover Z

Axio Observer Z1 microscope stand

Objectives

  • Fluar 5x/0.25 WD=12.5 M27
  • Fluar 10x/0.50 WD=1.9 M27
  • LD Plan-Neofluar 20x/0.4 Corr WD=8.4 M27 2,388.00 2,388.00
  • LD Plan-Neofluar 40x/0.6 Corr WD=3.3 M27 3,041.00 3,041.00
  • LD Plan-Neofluar 63x/0.75Corr WD=2.2 M27

Fluorescence:

  • X-cite fluorescence source
  • Dapi, Green, Texas Red filter cubes

Cameras:

  • Axiocam MRm firewire monochrome camera
  • AxioCam ICc1 color camera

Computer:

  • Imaging workstation
  • Windows7
  • AxioVision 4.8
  • Robosoftware 4.5
  • LCD-Pen display