Zeiss laser microdissection and capture

Duke LMCF laser capture Capabilities:
  • Cut and extract regions of a tissue
  • UV (355 nm) cutting beam
  • Non-contact capture
  • Also provides stitched imaging of large areas with fluorescence and color camera imaging

Location: Room 111 Research Park II (613-8219)

Cost: $21 /hr discounted for bulk use

User manuals: Manual for Laser capture (html)
Manual for image stitiching (html)

Request training | Reserve instrument

This system was purchased with NIH funding awarded to CHAVI and DHVI , who kindly donated the system to LMCF so all at Duke would be able to use this system and technology.

Information on how the system works

Detailed specifications

PALM MicroBeam 4.2

Robomover Z

Axio Observer Z1 microscope stand

Objectives

  • 5X/0.12 Fluar, 420130-9900: NA:0.12, air, WD: 10.1mm, coverglass: 0.17mm, Thread type: M27x0.75
  • 10x/0.25, Fluar, 20140-9900 NA:0.25, air, WD: 4.5mm, coverglass: 0.17mm, Thread type: M27x0.75
  • 20x/0.4, Korr LD Plan-Neofluar, 21350-9970: NA:0.40, air, WD: 7.9mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.75
  • 40x/0.60, Korr LD Plan-Neofluar, 21360-9970: NA:0.60, air, WD: 2.9mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.75
  • 63x/0.75 Korr LD Plan-Neofluar, 421380-9970: NA:0.75, air, WD: 1.7mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.7

Pixel sizes in micrometers for the AxioCam ICc1 (Color camera)
5x: 1.48; 10x: 0.74; 20x: 0.37; 40x: 0.18, and 63x: 0.12

Pixel sizes in micrometers for the AxioCam MRm (Mono-chrome camera)
5x: 1.29; 10x: 0.65; 20x: 0.32; 40x: 0.16, and 63x: 0.10

Fluorescence:

  • X-cite fluorescence source
  • Dapi, Green, Texas Red filter cubes

Cameras:

  • Axiocam MRm firewire monochrome camera
  • AxioCam ICc1 color camera

Computer:

  • Imaging workstation - HPZ840, 32 GB RAM
  • Windows7 64bit
  • AxioVision 64bit
  • Robosoftware 4.5
  • LCD-Pen display