Zeiss Laser Microdissection & Capture

Zeiss Laser Microdissection & Capture

User Manual(s)

Location

MSRB3 - 4223

Capabilities

  • Cut and extract regions of a tissue
  • UV (355 nm) cutting beam
  • Non-contact capture
  • Also provides stitched imaging of large areas with fluorescence and color camera imaging

Cost

$28 per hour

Detailed Specifications

This system was purchased with NIH funding awarded to CHAVI and DHVI , who kindly donated the system to LMCF so all at Duke would be able to use this system and technology.

Information on how the system works (pdf)

PALM MicroBeam 4.2

Robomover Z

Axio Observer Z1 microscope stand

Objectives

  • 5X/0.12 Fluar, 420130-9900: NA:0.12, air, WD: 10.1mm, coverglass: 0.17mm, Thread type: M27x0.75
  • 10x/0.25, Fluar, 20140-9900 NA:0.25, air, WD: 4.5mm, coverglass: 0.17mm, Thread type: M27x0.75
  • 20x/0.4, Korr LD Plan-Neofluar, 21350-9970: NA:0.40, air, WD: 7.9mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.75
  • 40x/0.60, Korr LD Plan-Neofluar, 21360-9970: NA:0.60, air, WD: 2.9mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.75
  • 63x/0.75 Korr LD Plan-Neofluar, 421380-9970: NA:0.75, air, WD: 1.7mm at coverglass 0.75, coverglass: 0-1.5mm, Thread type: M27x0.7

Scale (micrometers/pixel):

AxioCam ICc3 (Color camera)
5x: 1.10; 10x: 0.55; 20x: 0.27; 40x: 0.14, and 63x: 0.09

AxioCam MRm (Mono-chrome camera)
5x: 1.29; 10x: 0.65; 20x: 0.32; 40x: 0.16, and 63x: 0.10

Fluorescence:

  • X-cite fluorescence source
  • Dapi, Green, Texas Red filter cubes

Cameras:

  • Axiocam MRm firewire monochrome camera (1388*1040 = 1.4 MP)
  • AxioCam ICc3 color camera (2080*1540 = 3.3 MP)

Computer:

  • Imaging workstation - HPZ840, 32 GB RAM
  • Windows7 64bit
  • AxioVision 64bit, Rel 4.8
  • PALMRobo 4.6
  • LCD-Pen display