A Basic introduction to microscopy: Introduction Microscope configurations Transmitted light Fluorescence Fluorescence microscopes Stereoscopes Confocals Spinning disk confocals Two photon excitation TIRF Preparing your samples Objectives Images Deconvolution Image analysis Other points/FAQs |
Transmitted light You've probably done this type of microscopy looking at tissue culture cells. In brightfield, light passes through the specimen, some is absorbed/scattered and an image is formed from what passes through. Contrast is improved with techniques such as phase contrast or DIC. Tissue samples can be stained with dyes (eg H&E) or with immuno-histochemistry and colour images can be captured on either the Axio Imager or the stereoscope.
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