Olympus FV1000 Multiphoton
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Capabilities:
Location: 112 Research Park II (613-8219) Cost: $41.20/hour base rate, discounted for bulk use |
The system is to be upgraded with a ScaleView lens and motorized stage. The scaleview objective is optimized for imaging samples optically cleared in the scaleview reagent. Sample are fixed and cleared and the fluorescent proteins expressed in the original tissue can be imaged to a depth of up to 8 mm. The motorized stage will allow 3D tiling and assembly of data sets covering large areas and volumes of tissue. This website has examples of the images that can acquired.
| This system was purchased partially with a shared instrumentation grant for a system for intravital imaging. Users with such experiments receive booking priority on this system in that they may book up to 14 days ahead. All other users with fixed or other live samples are very welcome to use spare time on the system. We will review this policy as the use of the system develops to ensure the system serves its intended use and also more general use in the most efficient manner. |
Detailed specifications
Laser:
- Chameleon Vision II single box Ti:Saph fsec laser with pulse compensation
- Tunable range 680-1080 nm @40 nm/sec, 80 MHz rep rate, 140 fsec pulse width
- 0-47,000 fsec2 units of dispersion compensation
- Link to Two-photon cross sections at Cornell | MPE spectra from Olympus
Excitation beam optics:
- Intensity control via AOM
- Automatic beam expander
- Gold-coated scanning mirrors
- FV1000 MPE Exclusive scanhead
Microscope:
- Olympus BX61WI upright fixed stage
- 25x/1.05 NA water objective optimized for MPE XLPL25XWMP WD 2.0 mm 0 to 0.23 micron coverslip correction
- 25x/0.9 NA ScaleView immersion lens XLPLN25XSVMP WD 8 mm 0 to 0.23 micron coverslip correction
- Blue, Green and Red standard fluorecence cubes
- C-mount with documentation camera available for widefield fluorescence or brightfield
- Versatile stage with spacer to give large free working distance under objective (98 mm)
Detectors:
- 4 high efficiency non-descanned detectors in the epi position
- Emission bands available - Dapi, CFP,GFP, YFP, Red in switchable cubes
Incubation/perfusion system available:
- Warner instuments heated stage top chamber
- Dual channel heater controller - chamber and solution
- Perfusion system