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Equipment
The Facility has a wide variety of microscopes of various
configuations and modalities allowing users to image a large
range of samples on microscopes optimal for
the needs of the sample being imaged. LMCF staff
can advise you on the best choice for your experiments.
Leica SP5 confocal microscope
- Inverted microscope
- 405, Ar/2, 561, 594, 633 laser lines
- AOBS based system
- Conventional and resonant scanners
- Three Spectral detectors
- 10x to 100x objectives (dry, water, oil)
- Environmental chamber for live cells
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Zeiss LSM 510 upright confocal microscope
- Generally reserved
for experiments where a live specimen is imaged over a period
of several hours.
- Upright microscope
- Water objectives ideal for living specimens
- UV, Ar/2, 543, 633 laser lines
- Heated stage insert
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Two
photon microscope- Objective inverter
- Tunable Chameleon laser
- Pulse compensation
- Two non-descanned detectors
- Three descanned detectors
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Leica AM TIRF MC system
- TIRF imaging
- Automated alignment
- Variable TIRF depth
- 4 channels
- EM-CCD camera
- Environmental chamber
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Live cell station
- Inverted Zeiss Axio Observer microscope
- Environmental chamber for live cells
- Filter sets for all fluorescent proteins
- 10x to 100x objectives (dry, oil)
- EMCCD and high resolution CCD cameras
- Scanning stage - multiposition time lapse
- MetaMorph 7.5 software
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Zeiss Lumar.V12 stereoscope
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High performance stereoscope
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Fluorescence, transmitted and reflected light
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Red and Green fluorescence filters
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Digital cameras (colour and fluorescence) with AxioVision 4.6 image
acquisition software
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Microinjection system
- Capable of injecting up to 100 pL DNA, antibody, proteins, inhibitors etc into
adherent cells
- Eppendorf FemtoJet/TransferMan NK2 microinjection system
- Olympus IX-71 inverted fluorescence microscope
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Image analysis workstations
- Two dedicated workstations are available for
analysis and processing of data.
- Software available - MetaMorph,
Huygens SVI deconvolution, ImageJ, Zeiss LSM Image Examiner,
Photoshop.
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| Electron Microscopy available at Duke:
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Materials microscopes in SMIF
LMCF
works closely with SMIF to provide microscopes optimized for materials/engineering applications. Capabilities available in SMIF include:
- Reflected
light brightfield, darkfield and DIC
- Fluorescence
- Digital image capture and measurement
- Dust-free environment
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Imaging Centers and Institutes at Duke University
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