Duke University Light Microscopy Core Facility

The Facility has a wide variety of microscopes of various configuations and modalities allowing users to image a large range of samples on microscopes optimal for the needs of the sample being imaged. LMCF staff can advise you on the best choice for your experiments.

Leica SP5 confocal microscope Inverted microscope 405, Ar/2, 561, 594, 633 laser lines AOBS based system Conventional and resonant scanners Three Spectral detectors 10x to 100x objectives (dry, water, oil) Environmental chamber for live cells	Zeiss LSM 510 inverted confocal microscope Inverted microscope 405, Ar/2, 561, 594, 633 lasers 10x dry to 100x oil objectives Ideal for multicolour imaging of fixed samples
Zeiss LSM 510 upright confocal microscope Generally reserved for experiments where a live specimen is imaged over a period of several hours. Upright microscope Water objectives ideal for living specimens UV, Ar/2, 543, 633 laser lines Heated stage insert	Two photon microscope Objective inverter Tunable Chameleon laser Pulse compensation Two non-descanned detectors Three descanned detectors
Spinning disk confocal microscope Inverted Olympus IX-70 microscope Kr-Ar Laser - 488, 568, 647 10x dry to 100x oil objectives MetaMorph 7.5 software	Leica AM TIRF MC system TIRF imaging Automated alignment Variable TIRF depth 4 channels EM-CCD camera Environmental chamber
Zeiss Axio Observer widefield fluorescence microscope Inverted microscope Phase contrast and DIC Filter sets for wide variety of fluorophores 10x dry to 100x oil objectives MetaMorph 7.5 software	Zeiss Axio Imager widefield fluorescence microscope Upright microscope Filter sets for wide variety of fluorophores 10x dry to 100x oil objectives Stepper for Z-stacks Colour camera for histology slides MetaMorph 7.5 software
Live cell station Inverted Zeiss Axio Observer microscope Environmental chamber for live cells Filter sets for all fluorescent proteins 10x to 100x objectives (dry, oil) EMCCD and high resolution CCD cameras Scanning stage - multiposition time lapse MetaMorph 7.5 software	Zeiss Lumar.V12 stereoscope High performance stereoscope Fluorescence, transmitted and reflected light Red and Green fluorescence filters Digital cameras (colour and fluorescence) with AxioVision 4.6 image acquisition software
Microinjection system Capable of injecting up to 100 pL DNA, antibody, proteins, inhibitors etc into adherent cells Eppendorf FemtoJet/TransferMan NK2 microinjection system Olympus IX-71 inverted fluorescence microscope	Image analysis workstations Two dedicated workstations are available for analysis and processing of data. Software available - MetaMorph, Huygens SVI deconvolution, ImageJ, Zeiss LSM Image Examiner, Photoshop.

Electron Microscopy available at Duke:

High resolution SEM, TEM and AFM are available at the Shared Materials Instrumentation Facility (SMIF). Please contact SMIF to use any of these resources.
Environmental SEM is available at the Biology Department's Facility.

Materials microscopes in SMIF

LMCF works closely with SMIF to provide microscopes optimized for materials/engineering applications. Capabilities available in SMIF include:

Reflected light brightfield, darkfield and DIC
Fluorescence
Digital image capture and measurement
Dust-free environment

Imaging Centers and Institutes at Duke University